University:
Eastern Kentucky University
Course:
BIO 315 | Genetics
Academic year:

2020
Views:

498

Pages:

1
Author:

Isabella Bolton

NAME Mutations Worksheet Deletion, Insertion & Substitution here are several types of mutation: DELETION (a base is lost/deleted) ➤ INSERTION (an extra base is added/inserted) --Deletion & insertion may cause what's called a FRAMESHIFT mutation, meaning the reading "frame" changes, thus changing the amino acid sequence from this point forward ➤ SUBSTITUTION (one base is substituted for another) ---If a substitution changes the amino acid, it's called a MISSENSE mutation If a substitution does not change the amino acid, it's called a SILENT mutation If a substitution changes the amino acid to a "stop," it's called a NONSENSE mutation Complete the boxes below. Classify each as Deletion, Insertion or Substitution AND as either frameshift, missense, silent or nonsense (Hint: Deletion & Insertion will always be frameshift). Original DNA Sequence: TACACCTTGGCGACCACT... mRNA Sequence: AUGUGGAACKGCUGCUGA transcribe Amino Acid Sequence: med try ash.arg.cys. stop. Mutated DNA Sequence #I What's the TACATCTTGGCGACGACT... mRNA sequence? AUGUAGAACCGLUGCUGA (Circle the change) hat will be the amino acid sequence? met. Stop Will there likely be effects? yes! What type of mutation is this? nonsence Mutated DNA Sequence #2 TACGACCTT TGGC CGACGACT... (Circle the change) What's the mRNA sequence? AUGOUG/GAACCGILUGICUGIA What will be the amino acid sequence? met-leu- Glu-pro-Lev-lev Will there likely be effects? yes What type of mutation is this? Insertion Mutated DNA Sequence #3 TACACCTTAGCGACGACT... What's the mRNA sequence? AUGUGGAAN GEUGCUGA (Circle the change) What will be the amino acid sequence? med try-aspary-cus-stop Will there likely be effects? no What type of mutation is this? Silent Mutated DNA Sequence #4 TACACCTTGGCGACTACT... hat's the mRNA sequence? AUGUGGAACCGCUGAUGA What will be the amino acid sequence? met try-aspary-stop Will there likely be effects? yes. What type of mutation is this? nonsense (Circle the change