University:
Eastern Kentucky University
Course:
CHE 431 | Metabolic Biochemistry
Academic year:

2020
Views:

48

Pages:

1
Author:

Kaiden Murphy

Appendix L: Glycolysi Glucose has 6 carbons Glycolysis Occurs in cytoplasm Pyruvate has 3 carbons. Glycolysis produces two Pyruvates STEPS Glucose Hexokinase ATP Induced fit Regulated by G 6-P feedback ADP 1 Glucose 6-phosphate 2 Phosphoglucose isomerase Fructose 6-phosphate Phosphofructokinase (PFK) ATP Committed step Regulated by ATP/AMP ratio and Citrate ADP 3 Fructose 1,6-bisphosphate Aldolase Dihydroxyacetone phosphate Triose phosphate isomerase 5 4 Glyceraldehyde 3-phosphate Glyceraldehyde 3-phosphate dehydrogenase + Pi, NAD NADH 6 1,3-Bisphosphoglycerate Phosphoglycerate kinase ADP ATP 7 3-Phosphoglycerate 8 Phosphoglycerate mutase 2-Phosphoglycerate Note: PEP is high energy because the phosphoryl group traps PEP in its enol form. When the phosphoryl group is donated to ADP, making ATP, the enol converts to a more stable ketone (pyruvate), which releases a lot of energy. Reactants Products 1 Glucose 2 ATP 4 ADP 2 NAD+ 2 Pi 2 Pyruvate 2 ADP 4 ATP (2 net gain) 2 NADH 2 H+ 2 H 2O Enolase H2O 9 Phosphoenolpyruvate (PEP) Pyruvate Kinase ADP Regulated by covalent modification. Phosphorylated = inactive !Alanine = inactive ATP 10 The Fate of Pyruvate Pyruvate Cori Cycle Citric Acid Cycle