The Cow Eye Dissection Lab

Name ________ Date _2/1/2021___________ Period _____ Score out of 25 _____ Corrected by ___________________________ The Cow Eye Dissection Lab What are the structures of the mammalian eye and how do they function? The mammalian eye consists of many specialized cells and tissues that make up several different structures. The structures have certain functions and together, they form images that are interpreted by the brain. In this investigation, you identify the structures of a cow eye and learn their functions. • Materials • Safety goggles • Preserved cow’s eye • Apron or tee-shirt • Dissection scissors • Dissection tray • Forceps • Vinyl or latex gloves External features of the eye A. Locate the cornea, sclera, and optic nerve. a. The white part of the eye, the sclera, is a tough, outer covering of the eyeball. The sclera gives the eye its shape and helps to protect the delicate inner parts. b. The blue covering over the front of the eye is the cornea. When the cow was alive, the cornea was clear. Together with the lens, the cornea refracts light and helps the eye to focus. The cornea gives a larger contribution to the total refraction than the lens. The curvature of the cornea is fixed while that of the lens is changeable. In your cow’s eye, the cornea may be cloudy. c. You may be able to look through the cornea and see the iris, the colored part of the eye, and the pupil, the dark oval in the middle of the iris. At the back of the eye is the optic nerve. To see the separate fibers that make up the optic nerve, pinch the nerve with a pair of scissors or your fingers. If you squeeze the optic nerve, you may get some white goop. That is myelin, the fatty layer that surrounds each fiber of the nerve. It is the nerve that transmits visual information from the eye to the brain. 1. List two functions of the sclera. The sclera gives the eye its shape and it also helps to protect the delicate inner parts. 2. The cornea covers and helps protect the eye. List two other functions of the cornea. The cornea refracts light and helps the eye to focus. 3. What is the function of the optic nerve? The nerve transmits visual information from the eye to the brain. Dissection probes • • Paper towels Plastic trash bag B. Examine the fat and muscle surrounding the eyeball. a. Without moving your head, look up. Look down. Look all around. Six muscles attached to your eyeball move your eye so you can look in different directions. Cows have only four muscles that control their eyes. They can look up, down, left, and right, but they can’t roll their eyes like you can. Locate the externally attached muscles. These muscles control eye movement and help focus images. b. Although the muscles of each individual eye work as a team, the eyes themselves do not focus or work together until months after birth. However, one eye remains dominant. Form a circle with your thumb and index finger. Hold that position and place your hand in front of you. With both eyes, look at something through the circle. Continue to hold that position and close one eye; then open it. Close the other eye. The eye which is still able to view the object through the circle is your dominant eye. c. If you reach up and feel around your eye, you’ll feel the bone of your skull. There’s yellow fat surrounding your eyeball to keep it from bumping up against the bone and getting bruised. 4. What is the function of the externally attached muscles? - Six muscles attach to our eyeballs which move our eyes so we can look in different directions. 5. Which of your eyes is dominant? - My left eye is dominant. 6. What is the purpose of the layer of fat? - The yellow layer of fat surrounding our eyeballs keep them from bumping up against the bones and getting damaged and bruised. C. Cut away the fat and muscle. D. Remove the cornea – Use the photos on the placemat to help you. a. Use a scalpel to make an incision in the cornea. (Careful — don’t cut yourself!) Cut until the clear liquid under the cornea is released. That clear liquid is the aqueous humor. It’s made of mostly of water and keeps the shape of the cornea. b. Use the scalpel to make an incision (cut) through the sclera in the middle of the eye. c. Use your scissors to cut around the middle of the eye, cutting the eye in half. You’ll end up with two halves. On the front half will be the cornea. The cornea is made of pretty tough stuff—it helps protect your eye. It also helps you see by bending the light that comes into your eye. d. Once you have removed the cornea, place it on the board (or cutting surface) and cut it with your scalpel or razor. Listen. Hear the crunch? That’s the sound of the scalpel crunching through layers of clear tissue. The cow’s cornea has many layers to make it thick and strong. When the cow is grazing, blades of grass may poke the cow’s eye—but the cornea protects the inner eye. E. The next step is to pull out the iris. a. The iris is between the cornea and the lens. It may be stuck to the cornea or it may have stayed with the back of the eye. Find the iris and pull it out. It should come out in one piece. b. You can see that there’s a hole in the center of the iris. That’s the pupil, the hole that lets light into the eye. The iris contracts or expands to change the size of the pupil. In dim light, the pupil opens wide to let light in. In bright light, the pupil shuts down to block light out. c. The back of the eye is filled with a clear jelly. That’s the vitreous humor, a mixture of protein and water. It’s clear so light can pass through it. It also helps the eyeball maintain its shape. The vitreous humor is attached to the lens. d. F. Now you want to remove the lens. a. It’s a clear lump about the size and shape of a squashed marble. The lens is a transparent structure in the eye that, along with the cornea, helps to refract and focus light. b. A ring of tiny ciliary muscles, located along the inner side of the iris, connects the lens to the middle layer of the eye. Ciliary muscles contract to change the curvature of the lens. c. The lens of the cow’s eye feels soft on the outside and hard in the middle. Hold the lens up and look through it. In a living organism, it is completely transparent. (You cow lens may not be transparent.) To focus on closer objects, it gets fatter so it can refract more light. d. Put the lens down on a newspaper and look through it at the words on the page. If your lens is transparent, it should magnify. Name ________ Date ____2/1/21_____________ Period______ Cow’s Eye Dissection Lab Page 2 7. List the two functions of the lens. The lens is a transparent structure in the eye that, along with the cornea, helps to refract and focus light. To help us focus on closer objects is gets fatter to refract more light and vice versa for focusing on objects further away. 8. Describe the iris and explain its function. The iris is the colorful part of the eye. The iris is between the cornea and the lens and has a hole in the middle for the pupil. The iris contracts or expands to change the size of the pupil. In the dim light, the pupil opens wide to let light in. In bright light, the pupil shuts down to block light out. 9. Describe the pupil. Pupils are the black center of the eye. Their function is to let in light and focus it on the retina (the nerve cells at the back of the eye) so you can see. Muscles located in your iris (the colored part of your eye) control each pupil. In humans, the pupil is normally round while in cows it is oval. 10. If you enter a very bright room after being in the dark, what would happen to your pupils – get larger or get smaller? In bright light, your pupils get smaller (constrict) to limit the amount of light that enters. In the dark, your pupils get bigger (dilate). That allows more light in, which improves night vision. This action doesn’t happen instantly and so there is an adjustment period when going from a very bright or very dark room to the other. G. Now it’s time to examine the retina. a. If the vitreous humor is still in the eyeball, empty it out. b. On the inside of the back half of the eyeball, you can see some blood vessels that are part of a thin fleshy film. That film is the retina. Before you cut the eye open, the vitreous humor pushed against the retina so that it lay flat on the back of the eye. It may be all pushed together in a wad now. c. The retina is made of cells that can detect light. The eye’s lens uses the light that comes into the eye to make an image, a picture made of light. That image lands on the retina. The cells of the retina react to the light that falls on them and send messages to the brain. d. Use your finger to push the retina around. The retina is attached to the back of the eye at just one spot. Can you find that spot? That’s the place where nerves from all the cells in the retina come together. All these nerves go out the back of the eye, forming the optic nerve, the bundle of nerves that carries messages from the eye to the brain. The brain uses information from the retina to make a mental picture of the world. e. The spot where the retina is attached to the back of the eye is called the blind spot. Because there are no light-sensitive cells (photoreceptors) at that spot, you can’t see anything that lands in that place on the retina. 11. Why does the optic nerve cause a blind spot? (2 points) Be specific. The blind spot is the location on the retina known as the optic disk where the optic nerve fibers exit the back of the eye. It is also where the blood vessels enter the eye. This approximately 1.5mm area has no rods or cones and because of these reasons we have a tiny blind spot in our vision, but thankfully unless we are trying we do not notice it. H. Check out the tapetum. a. Under the retina, the back of the eye is covered with shiny, bluegreen stuff. This is the tapetum. It reflects light from the back of the eye. Have you ever seen a cat’s eyes shining in the headlights of a car? Cats, like cows, have a tapetum. A cat’s eye seems to glow because the cat’s tapetum is reflecting light. If you shine a light at a cow at night, the cow’s eyes will shine with a blue-green light because the light reflects from the tapetum. Humans do not have this tapetum. I. Find your blind spot. a. To find your blind spot, use the two dots below. Hold one hand over your left eye and look directly at the left-hand dot. At first, you can see both dots even though you're looking directly at only one. As you slowly move the page closer to your eyes, the right-hand dot disappears! If you move your eye, the dot will reappear, but as long as you focus on the first dot, the second will be invisible. Move even closer and the missing dot reappears. You’ve found your blind spot! NAME: DATE: 2/1/21 12. Following lab instructions and Cleaning up – 2 points ________________________ Teacher initials Match each structure of the cow eye with its function by writing the letter. m Eye structure Function 13. __E___ External muscles A. A clear structure that refracts light and can change in curvature 14. __H___ Retina B. A tiny ring of muscles that change the shape of the lens 15. __D___ Cornea C. The pigmented ring of muscles that change the size of the pupil 16. __A___ Lens 17. __F___ Optic nerve 18. __C___ Iris 19. __G___ Sclera 20. __B___ Ciliary muscles D. Works with the lens to refract light and helps the eye to focus E. Move the eye around F. Transmits signals from the eye to the brain G. Gives the eye its shape and protects the inner parts H. A thin layer of cells that convert light into nerve signals 21. Name two physical differences between the cow eye and the human eye. (2 points) Two physical differences between a cow eye and a human eye: The cow's pupil is oval while a human’s pupil is round. Also, a cow’s eyeball doesn’t have as many movement muscles surrounding it as a human’s eyeball does. So cows can only move their eyes up down left and right, but not all around like a human can, which prevents them from being able to roll their eyes. 22. Paper is neatly written, no words crossed out, and words easily read.