the 20 Nome Spichuen represent the chanel of os inus and Concand on fr mell The Spectre also called 8J Spectra. I types lomonuclear ID I resched NPIR bensten & I treguney, bervers / Sominatius and it 0. represented as and the سه 2 Hedonly of the Agent and indorty of the Signed represented by ignoring Called on the inkonty of the Signt is ID NMR Spechan due to strong irradiation of RF I the d. KB Spin states population ↑ and the energy is toansfered to develtly attached Bc. P In one of the 20 HNMR Experiment which verulti In a 2D NMR spectruem. In the Spectrewn F₂ axis represents the chemical shift shift of 17 Nuclei and Fi ands coupling constant ie 'JIBCH repocents Heteronuclear principle: It involves focer steps. In the prepart IRF radiat with strong pulx is paned through the 'H channel, decoupler is on. So that they will NOE effect on Bc Nuclei At the end of the peopcuat? maple) of step It RF radiation 90°x pele is passed through the Be channel then the Excited Be Nuclei precess freely along Y-ox's Then in the X-4 plare of ostating frame the Bc Muchi are in xcited state and in a Suitable position for the coupling interaction with directly attached it nuclei In the evoluts period the decoupler the magnetisat transfer form directly attached 't Mules to Be Nuclei. At the half time of evolut 180° RF pulse is pared along y-axis. After Evaristor Home and Mixing period FID's are 1BC channel. This Exp is repeated by gradual ty Evolution time. The FID's undergo doo double Fourier tranfpem over to and then ti 2J resolved spectrum to give heteronew in this spectroscopy the Exp gives the 20 NMR spectrum in which the chemical shift along both frequency onts fo awell as fi are corelated with exces othes and gries the informatn regourding types of magnetic nuder and their coupling known as Inwaution. This technique. Es correlated 2D NMR technique and this gives in formation regarding interrelation biw dys nudei

D NMR spectroscopy

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