Isolation and Growth of Mold in Food Fungi or fungi are one example of a frequent multicellular cell we encounter in our daily lives. Both in the form of macro fungi such as oyster mushrooms, button mushrooms or ear mushrooms and many more or as micro fungi. Fungi divided into groups Ascomycota, Basidiomycota, Zygomycota, Chytridiomycota and some belong to the Deuteromycota group. Ascomycota mostly are microfungi, basidiomycota are mostly macrofungi, zygomycota are not Having septa and hyphae, chytrids are generally aquatic fungi and deuteromycota which are usually called anamorphic fungi. The morphology of fungi has hyphae and septum which works later for fungal growth B. Materials and Tools Foodstuffs:         Peanuts 10 grams Tempe Tempeh yeast PDA (Potato Dextrose Agar) SDA (Saboraud Dextrose Agar) 0.05 chloramphenicol MEA (malt extract agar) Diluent solution Tool :        Autoclave Incubator Laru/use tempe Vortex Spirit lamp Micropipette Tip C. Work procedures C.1. For nuts 1. Prepare SDA and SDB (Sobaroud Dextrose Agar and Broth) which have been given with chloramphenicol 0.05% of the media volume 2. Prepare a physiological NaCl diluent solution of 1 tube containing 90 ml and solution diluent contains 9 ml. Sterilize and cool 3. Meanwhile, prepare the material to be tested by crushing the material with mortar or pestle, the pestle mortar tool is first sprayed with alcohol 95% 4. Take 10 grams of the ingredients, put them in 90 ml of diluent, then shake until evenly mixed. Then from the 90 ml diluent solution, take 1 ml votex and do it, then put it in a diluent solution of 9 ml to 102 in a sterile and aseptic manner 5. Use the pour plating method or pour method using SDA 6. Incubate at 29-30o C for 4-5 days (room temperature) 7. Do this by shaking and letting it sit C.2. For Food Ingredients: 1. Prepare MEA, TEA and PDA media, sterilize and cool 2. Prepare 1 tube of 90 mL diluent solution and 3 tubes of 9 mL sterile diluent solution 3. Take and weigh a 10 gram sample and crush it in a mortar with a pestle have been sprayed with alcohol first. Then put it in diluent 1 mL and dilute to 10-3 , vortex, and do it sterilely and aseptic 4. Take 1 mL of food with a micropipette and put it in a petri dish and pour in the available media 5. Incubate at room temperature for 5 days (29-30o C) and record the results C.3. For laru/usar tempeh and tempeh yeast 1. Prepare SDB media and 9 mL diluent solution and sterilize 2. Take 1 gram of laru/usar tempeh or tempeh yeast and put it in the solution diluent, vortex 3. Then take 1 mL and put it in the prepared SDB and incubate by shaking for 5 days 4. Observe the results Creating Culture Slides for Mold 1. Prepare hard sterile agar (PDA) in a petri dish 2. Cut a square and put it in a sterile petri dish that has been coated with filter paper and a U-shaped glass rod 3. Use a needle in succession to cut the rectangle and pierce it on the 4 sides of the agar 4. Cover with a cover glass, and drip with sterile distilled water on filter paper the 5. Incubate for 4-5 days at room temperature 6. Can be seen under a microscope by taking a cover glass and dripping on it with previous lactophenol blue stain.