Salmonella-Shigella Detection Test in Food Ingredients B. Materials and Tools Ingredients:        Tetrahionate broth atau Rappaport vasidialis broth XLD ( Xylose lysine deoxycholate agar) BSA (Bismuth Sulfite Agar) HEA (Hektoen Enteric Agar) TSIA (Triple Sugar Iron Agar) LIA (lysine iron agar) Sample:       Gado-gado Ketoprak Rendang Counts It's crime Beef jerky Tool :       Blender Analytical Balance Sheet Bunsen Micropipette Tips Erlenmeyer Vortex C. Procedure 1. Weigh 10 grams of sample and place it in sterile 90 diluent solution mL. Then do the enrichment stage. 2. The enrichment stage is carried out by inoculating 1 mL of sample each into 9 mL of Tetrahionate Broth or Rappaport Vasidialis and incubated for 24 hours at 37oC . 3. The estimation test is carried out by taking one loop from the culture enrichment and etched onto SS agar. After incubation for 24-48 hours, view the presence of Salmonella/Shigella colonies, namely in the form of cloudy or clear colonies or not colored. the middle is black. In BSA, colony. In HEA usually there with black centers or typical On XLD agar media it is pink with or without these are brown colonies, gray colonies or black are blue, greenish blue colonies with or without colonies. 4. The reinforcement test is carried out by taking two separate colonies showing typical colonies of Salmonella or Shigella and inoculated on agar slants TSIA by making scratches on the surface so that it is slanted, then stick it into the bottom of the agar and stick it into the LIA upright agar. Incubation TSI agar and LI agar were carried out at 37o C for 24-48 hours. Match with table to see the presence of Salmonella or Shigella bacteria. As a scratch control culture Salmonella on SS/XLD/BSA/HEA agar and inoculate also on agar slants TSI and LIA to see the reaction that occurs. 5. Interpretation of Results for TSIA: TSIA media is used to determine the ability of germs to ferment carbohydrates, can also be used to determine the formation of H2S , namely seeing Do germs ferment methionine and cysteine (amino acids that have S group). In TSIA media there are the amino acids methionine and cysteine, if germs ferment these two amino acids, the S group will come out and the S group will come out combines with H2O to form H2S. Next H2S combines with Fe2+ forms black FeS and precipitates. a. Only ferments glucose: If the bottom of the medium is yellow (acidic) and the slant is red (alkaline) b. Ferment all carbohydrates: If the bottom of the media is yellow (acidic) and the slant is yellow (acidic) c. Does not ferment all carbohydrates: If the bottom (butt) of the media is colored red (alkaline) and the slope (slant) is red (alkaline) Fermentation in TSIA is also accompanied by the formation of CO2 gas which can be seen from rupture and lifting of agar.