Body Logistics 02: How to Examine Cells and Tissue (22 cards)

25/04/2024, 19:58 1. Anki Glossary: Semester 1 > [BL] Body Logistics > 02.How to examine cells and tissue (22 cards) What is tissue? Collection of cells that are adapted to form a specific function 2. Name the 4 different body tissues Epithelial, Connective, Muscle, Nerve. 3. What is limit of resolution/resolving power? The smallest distance between 2 objects where they can be distinguished as 2 separate objects. 4. Advantages of using Light microscopes Cheap to use, Fast preparation, Large field of view, Can view living and moving objects, Can see in natural colours. 5. Advantages of using Electron microscope Very high magnification (x500000), Very high resolution 6. Disadvantages of using Electron microscope Can only view dead inert objects, Dfficult expensive equipment and prep, Limited field of view, Only black and white pictures 7. Disadvantage of Light microscope (over electron Not as high magnification (x600 compared to microscope) x500000), Resolution is lower (but still good!) 8. What is Freeze fracture electron microscopy used To expose the plasma membrane for imaging. for? 9. How to do Freeze fracture Electron Microscopy? Freeze tissue to -160C and fracture by hitting with a knife edge. Fracture splits the membrane. Can be imaged What do we need to do to tissues before imaging Preserve (Formalin), Embed (Paraffin wax) in a with Light microscope? substance so it can be cut thin, Stain Give chemical names for each step (haematoxylin & Eosin) 11. What chemical do we use to preserve tissues? Formalin 12. What do we use to embed tissues so it can be cut Melted Paraffin Wax (solidifies when cooled) 10. down thin? 13. What 2 stains do we use for staining tissues? Haematoxylin and Eosin 14. What is a frozen section of tissue? Surgical specimen on a metal disc which is frozen rapidly to -25C and is solid. file:///E:/Program Files/Anki/Exports/Semester 1__[BL] Body Logistics__02.How to examine cells and tissue.html 1/2 25/04/2024, 19:58 15. Anki Glossary: Semester 1 > [BL] Body Logistics > 02.How to examine cells and tissue (22 cards) What advantages and uses of a Frozen section Much Much faster preparation of slides (10 mins vs rather than traditional histology technique? 6+ hours), but low quality slides. Used in intraoperatives consulation (requires a diagnosis/information fast) 16. What parts of a cell will Haematoxylin stain? Why? All Acidic structures. E.g Nucleus Because Haematoxylin is basic and reacts with acidic structures, making stain. 17. What parts of the cell will Eosin stain? Why? Basic structures. E.g cytoplasmic components As Eosin is acidic so will react with basic structures. 18. Why can't Haematoxylin and Eosin stain fat? The stains are water-soluble, not lipid-soluble 19. Advantages of using Formalin fixation over Frozen Higher quality section? Can be kept forever Disadvantages of using Formalin fixation rather Takes 6+ hours to prepare than frozen section? Formalin artifacts appear when dehydrated. 20. Lipids are removed in embedding process 21. 22. Disadvantges of using Frozen section rather than Low quality slides Formalin fixation? Can only keep for months, not forever What are the steps of preparing a biopsy for H&E Fixation staining? Embedding Cutting Staining file:///E:/Program Files/Anki/Exports/Semester 1__[BL] Body Logistics__02.How to examine cells and tissue.html 2/2