Examination Methods for Mosquito Body Surgery INTRODUCTION Location and shape to be dissected (section): 1. Salivary gland vesicles: Middle plate, lateral plate. 2. Digestive tract: Large stomach, malpigi, small intestine and rectum papilla. 3. Composition of the reproductive organs: Ovary, ovarian stem, ampilla and vagina. METHOD Wet pickling OBJECTIVE To examine and understand the process of dissecting the mosquito's body namely in the salivary glands,ovaries,and a big belly. TOOL a. Dissecting microscope b. Needle c. Glass objects d. Petridisk MATERIAL a. Chloroform b. NaCl 0.85% or Pz c. Mosquito PROCEDURE A. Salivary gland surgery 1. Mosquitoes are killed with chlorophom. 2. The legs and wings are cleaned or cut. 3. Drop physiological saline on the slide. 4. Place the mosquito on a glass object in a tilted or supine position. 5. The sexy needle in the left hand is inserted into the piston, and the sexy needle In the right hand, stab it into the head. 6. The head is drawn slowly in the physiological saline solution that has been prepared dripped. 7. If you pull it well, the salivary gland bubble will stick to it the neck of the head and the shape of the salivary glands will look like a banana and transparent. 8. It is possible that the salivary glands will break off and remain in the thorax, for This piston is pressed carefully in the physiological fluid until You can see the lobes of the salivary glands coming out of the thorax. 9. If the lobes of the salivary glands are visible, cut them and separate them from them dirt, clean from the remains of fur, skin and other dirt. 10. The horse's glands are dried, after drying they are fixed with methanol for approximately 1 minute, and dry again, once dry completely stained with gymsa solution (ADA below). 11. Examine under a microscope with a magnification of 5-6 x 100 times use immersion oil. NOTE: GIEMSA EXAMINATION METHOD: 1. Arrange the blood glass on the stain rack. Make a Giemsa solution with a ratio of 1 drop of Giemsa to 1 cc of buffer water 2. Pour the Giemsa solution over the blood preparation, and wait for 45 – 60 minute. 3. Wash with clean water until all the Giemsa liquid is removed, and dry. 4. Dry on a coloring rack in a slightly dry standing position. B. Large stomach surgery: 1. Mosquitoes are killed with chloroform 2. The legs and wings are cleaned (cut) 3. Drop physiological saline on the slide. 4. Place the mosquito on a glass object in a tilted or supine position under a dissecting microscope. 5. The sexy needle in the left hand is inserted into the thorax and the sexy needle In the right hand, stab into the seventh segment of the abdomen. 6. The seventh segment of the abdomen is gently drawn in physiological saline Here you will see a large stomach and ovaries. 7. Separate the large stomach and transfer it to another clean glass object drip physiological saline with 1% methylene blue (10 cc of physiological saline approximately 1 drop of methylene blue). 8. Cover the large stomach preparation with a glass lid and examine below microscope with low magnification. C. Surgery on the ovaries: 1. Mosquitoes that are dissected are mosquitoes that have an empty stomach or stomach contains blood. 2. From live mosquitoes to dissection, the method is the same as Mosquito large stomach surgery, only surgery on medium fluid. Ovarian surgery, does not use physiological saline fluid, but use distilled water or clean water. 3. If the ovaries have come out, then clean them of any remaining dirt later dried. 4. Examine under a microscope with weak magnification (5 x 10), after The ovaries are visible, replaced by magnification (5 x 40). 5. The ovaries are said to be parous (have laid eggs), if the trachioli end straight or loose. 6. The ovaries are said to be nulu parous (not yet laying eggs), if they have trachioli tight spiral shape. 7. The ovaries can be seen to have laid eggs 1 x, 2 x, 3 x. etc. Can be seen lots of bubbles in the dilatation.