Manufacturing Slide Mounting With Coloring METHOD Wet Preservation OBJECTIVE a. To identify and determine insect morphology. b. Colored to make it last longer. c. For collection. TOOLS a. Glass objects b. Cover glass c. Test tube d. Baker glass e. Petridisk dish f. Drop pipette MATERIAL Lice consist of human hair lice (Pediculus humanus)and fleas cat (Ctenocephalides felis). REAGENT a. NaOH or KOH 10% b. Alcohol 95% or 96% c. Alcohol 95% + Xylol (same ratio, 1: 1) d. Xylol e. Aquadest f. Acid fuchsin Method: 0.5 gram acid fuhsin plus 25 cc of 10% HCl and dissolved in 300 cc distilled water. PROCEDURE 1. Very small insects are inserted and their bodies are pigmented into the. 2. Test tube containing 10% NaOH. 3. Boil in a beaker or pan with boiling water for 1 – 10 hours or until color changes (10% NaOH becomes cloudy) and insects become transparent. So it depends on the thickness insect pigments but should not be allowed to dry. 4. Filter the insects with filter paper 5. Wash the insects with distilled water in a Petridisk twice. 6. Soak the insects in 95% alcohol (in a Petridisk) for 3 – 5 minute. 7. Soak the insects in fuhsin acid for 30 minutes. 8. Soak again in 95% alcohol for 3 minutes. 9. Put the insect in Ana alcohol (95% alcohol + xylol) for 3 minute 10. And finally soak the insects in xylol for 1 minute 11. Make a preparation with an object glass and cover glass then observe below microscope M = 10 12. If you have obtained a good shape of the insect under the microscope then attach the cover glass to the glass object using entellant.