Mounting Preparation Slides Without Coloring METHOD 1. Wet Preservation 2. Absolute need for chemicals OBJECTIVE 1. To preserve insects 2. For identification, know the morphology of the insect 3. For collection TOOL a. Cover glass b. Test tube c. Beaker glass d.Petri dish e. Drop pipette REAGENT a. KOH 10% / NaOH 10% b. Alcohol graded 30%, 50%, 70%, 95% or 96% c. Xylol d. Aquadest MATERIAL a. Cat Flea (Ctenocephalidesfelis) b. Lice (Pediculushumanus) c. Dog fleas (Ctenocephalidescanis) d. Fleas PROCEDURE A. CLEARING STAGE (THINNING) 1. Put the insect in a test tube containing 10% KOH or NaOH 10% 2. Boil in a beaker glass for 1 – 10 minutes until it boils thinning or transparency in insects. B. DRYING OR DEHYDRATION STAGE 1. Place the insect in a Petridisk containing alcohol graded with concentrations of 30%, 50%, 70%, 95% or 96% respectively each left for 5 minutes. Alcohol works for secrete fluids in the insect's body to make it last longer. 2. After that, put it in a Petridisk dish containing xylol for 1 minute. The goal is to clean the alcohol from stopping it drying drying. C. MOUNTING STAGE 1. Place the insect on a glass object, then look at it under a microscope. Observe whether any insects are damaged, then identify them. 2. Cover the preparation with a cover glass attached with entelan.