University:
Western Oregon University
Course:
BI 102 | General Biology
Academic year:

2022
Views:

23

Pages:

1
Author:

LauraParks11

General safety rules: 1- always wear a buttoned up lab coat, and closed shoes 2- tie back your hair 3- do not eat or drink in the lab, and do not get any personal items 4- keep your hands away from your face, and keep your work area clean and uncluttered 5- never touch, small, or taste the chemical (they are very dangerous) 6- remove spills immediately, and do not empty materials into the sink or trash can 7- dispose wastes accordingly in the yellow bucket 8- don’t fool around End of lab activity rules: 1- clean all lab equipment and return into place (make sure lids are back and containers are sealed tightly) 2- clean you space and place back your chair 3- wash your hands with water and soap Know all names and shapes! Microscope: an instrument used to see objects that are too small for the naked eyes Centrifuge: equipment that is driven by electric motor to separate the components of blood or other liquids Gel electrophoresis: a lab method used to separate mixtures of DNA and RNA molecules by size PCR machine: device used to amplify a piece of DNA Water bath: device used to incubate samples in water in order to maintain a constant temperature Micropipette: adjustable digital pipette lab tool used to transport and measure a specific volume of a liquid within its own range Operating a micropipette: 1- find the volume you want 7- pause 2- set the volume 8- withdraw the tip 3- attach the disposable tip 9- dispense the sample 4- depress the plunger to 1st stop 10- withdraw the pipette 5- immerse tip in sample 11- release the plunger 6- draw up sample 12- discard the tip Guidelines and precautions: - aim for immersion depth near the surface - aim for nearly vertical position - avoid all air bubbles and never lay the pipette on the side or invert it