University:
Western Oregon University
Course:
BI 102 | General Biology
Academic year:

2022
Views:

27

Pages:

1
Author:

LauraParks11

Microscope is an essential instrument used to see objects that are too small to be seen with the naked eye Electron microscope Light microscope Uses visible light and the image is seen in its original colors Compound 2-D image; the most commonly used microscope; you can view individual cells, since it has a high magnification and low resolution Uses beam of electrons and the image is seen in black and white; it has high magnification and resolution. Dissecting Scanning Transmission 3-D image; used for dissection and getting a better look at large specimen;can’t see individual cells; low magnification (Also called stereo microscope) 3-D image; specimen coated in gold; electrons bounce off; gives exterior view of specimen 2-D image; thin slices of specimen are obtained; electron beams pass through the specimen; gives interior view I 1 Ocular lens (eyepiece): lens at the top, the one you look through Tube: connects eyepiece to objective lenses Objective lenses: magnify the imagine; 3 or 4 on each microscope Revolving nosepiece: holds objective lenses and controls them Arm: supports tube and connects it to base Stage: flat platform where you place the slides Stage clips: holds slide on top of stage Diaphragm: adjusts intensity and size of light cone Illuminator (light source): light source used instead of mirror to see the specimen easily Coarse adjustment knob: raises and lowers stage Fine adjustment knob: fine-focusing and making the image clearer Base: at the bottom; supports microscope Determining total magnification: How to focus on specimen: 1- start with lowest power objective lens 2- while looking, raise the stage upward using the coarse adjustment knob until imagine is clear 3- click in the next power lens and do minor adjustments 4- continue with subsequent objective lens and fine focus only I