Blood Cells
• Common lineages of red and white cells
• Red cells from
– yolk sac blood islands (primitive lineage)
• only initial blood cells
• not self-renewing
– fetal liver (definitive lineage)
– adult bone marrow and spleen (definitive) Blood Cells Types Stem Cells
• Stem cells for self-renewing populations
• Several different ones
• How do you sort them out? Stem Cell: CFU-S
• Experiment 1
– X-irradiate mouse to kill stem
cells
– inject marked marrow cells from
donor
– each cell forms colony in spleen
(clone) = CFU-S
– include granulocytes,
macrophages, platelets,
erythrocytes, not lymphocytes Myeloid Lineage Stem Cell CFU-L
• Experiment 2
– inject marked marrow cells in mutant mouse
lacking all blood-forming cells
– rescues mouse and find circulating B and T
lymphocytes same abnormality as spleen
colonies: clone
– = CFU-L Lymphoid Lineage Stem Cell CFU-M,L
• Experiment 3
– Small number of cells (0.05% of marrow)
separated immunologically (unique
combination of surface molecules) can give rise
to both lymphoid and myeloid cells
– = CFU-M,L Red Cell Lineage
• Other cells more committed
– place bone marrow cells in culture with high or
low erythropoietin
– Low: small colonies (ca. 60 cells ca 5 div)
– High: large colonies (ca. 11-12 divisions)
– so CFU -> BFU-E (determined) -6-7 div->
– CFU-E (detm) -5div-> erythrocytes The BFU-E and CFU-E Criculatory System: Arteries
2 dorsal aortae
Umbilical artery
Vitelline artery
Nephric artery
Segmental arteries
2 ventral aortae (fuse) Human Aortic Arches
Mesoderm II Part 2
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