University:
Amherst College
Course:
BIOL 330 | Biochemical Principles of Life at the Molecular Level
Academic year:

2021
Views:

314

Pages:

14
Author:

Brent Cantu

Capitulo 5/6 1- Analyze the following table. Which of the following statements is true? 2- What are antibodies? 3- RNase is an enzyme that cleaves the P–O   ′ bond in RNA. It has two His residues in the 5 active site. Suggest a plausible explanation why the enzyme activity changes when pH is increased or decreased from pH 6.0, as shown in the graph. 4- Which of the statements about enzymes are true? 5- Which of the following statements is true regarding Immunoglobulins? 6- Considering the Steady State Model where [S] << KM, which of the following statements is true? 7- One way of expressing the rate at which an enzyme can catalyze a reaction is to state its turnover number. The turnover number is the maximum number of substrate molecules that can be acted on by one molecule of enzyme per unit of time. The table gives the turnover number of four representative enzymes. 8- If an Immunoglobulin binds to its antigen (where the antigen concentration is 6 x 10 -8  M) with a  𝜃 = 0.90. The Kd for this antibody for the antigen is: 7 x 10 -9 . 9- Are the given reactions examples of general acid/base catalysis, covalent catalysis, catalysis by approximation (proximity effect), or metal–ion catalysis? 10- If an enzyme has a kcat= 30 s-1 and a KM= 0.0050 M, and it is operating at a ¼ Vmax, the [S] is equal to: 11- Consider the enzymatically catalyzed reaction. 12- What kind of enzyme is catalyzing this reaction? 13- Consider the reaction. What effects are produced by an enzyme on the general reaction? 14- Which of the following statements is true with regards to MHC’s? 15- Enzymes are important molecules in biochemistry that catalyze reactions. The energy diagram illustrates the difference between a catalyzed reaction and an uncatalyzed reaction. Label the energy diagram. 16- Select the true statements for IgG: 17- Which interactions can contribute to the intrinsic binding energy during enzymatic catalysis? 18- Determine which of the graphs represents the relationship of reaction rate (velocity) and substrate concentration when the enzyme concentration of the non-allosteric enzyme is constant. 19- Considering the ELISA test, all the following statements are true. 20- Interleukins, a family of proteins secreted by the Helper T cells, are responsible for: 21- Which of the following statements is true for General Acid-Base Catalysis? 22- The energy of an electrostatic interaction between two charged atoms is dependent on the charges on the atoms, the distance between them, and the dielectric constant of the solvent. For example, the strength of a weak acid (𝐾a , acid dissociation constant) depends on the strength of the electrostatic interaction between a negatively charged carboxylic acid group and a proton. The solvent dielectric constant has a large influence on the p  𝐾  a for weak acids. 23- The rate equation for the second-order reaction of an enzyme (E) and its substrate (S) is 24- Which of the following statements is true for Metal ion catalysis? 25- Suppose that an arginine residue in the active site of an enzyme was mutated to alanine. As expected, the alanine mutant was inactive, suggesting that the arginine residue was critical to the catalytic mechanism.

Cheat Sheet - Final Biochem Part V

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