University:
Amherst College
Course:
BIOL 330 | Biochemical Principles of Life at the Molecular Level
Academic year:

2019
Views:

147

Pages:

16
Author:

Oliver Donovan

Capitulo 4/5 1- If the iron in Hb is in the +3 oxidation state, which of the following species is more likely to bind to the iron? 2- Which of the following statements is not true for the R state of Hb? 3- Hemoglobin Rainier (described by Adamson, Parer, and Stamatoyannopoulos in 1969) is a hemoglobin variant in which a His residue is substituted for Tyr at residue 145 of the β chain. This mutation destabilizes the T state. 4- Which five statements about hemoglobin and myoglobin structure are true? 5- Which of the following situations will shift the normal Hb curve towards the left? 6- Use the graph to estimate the dissociation constant, 𝐾d , and calculate the association constant, 𝐾aof the ligand. 7- Which ligand binds tightest? 8- Comparing NMR and X-ray diffraction techniques, one can say that: 9- As shown in the graph, hemoglobin exchanges oxygen and protons due to an inverse relationship between oxygen binding and proton binding known as the Bohr effect. The binding of oxygen to hemoglobin causes a conformational change that disrupts salt bridges, making oxyhemoglobin more acidic than deoxyhemoglobin. 10- The removal of 2,3-BPG from the Hb will cause the following: 11- Circular Dichroism: 12- Christen Anfinsen's experiment with RNAase suggested that the secondary and tertiary structure of the protein is determined by the aminoacid sequence. 13- There are almost 500 naturally occurring variants of hemoglobin. Most are the result of a single amino acid substitution in a globin polypeptide chain. Some variants produce clinical illness, though not all variants have deleterious effects. 14- Which of the following conditions changes the Kd of Hb. 15- Which of the following statements is true with regards to Chaperones/Chaperonins and HSP's? 16- The P50 of Hb is about 10 times larger than that of Mb. 17- Which of the following is true for Mb and Hb? 18- The graph is a Hill plot showing the binding of oxygen to myoglobin and hemoglobin. Label the graph and answer the two questions. 19- Which of the following statements is true regarding X-ray Diffraction? 20- Consider the binding of a protein to a ligand. If the [ligand] is 10 -7 M, what is the Kd when the percentage occupancy (or fractional saturation) is 40%. 21- Studies of oxygen transport in pregnant mammals show that the O  2 -saturation curves of fetal (HbF) and maternal (HbA) hemoglobin are markedly different when measured under the same conditions. 22- The mutated form of hemoglobin (hemoglobin S, or HbS) in sickle-cell anemia results from the replacement of a glutamate residue by a valine residue at position 6   in the β chain of the protein. Normal hemoglobin is designated H 23- In addition to transporting oxygen, hemoglobin also helps maintain a constant blood pH   of around 7.4. 24- Which of the following statements is true with regards to the substitution of a  𝛾 (gamma) subunit instead of a  𝛽 (beta), in fetalHb? 25- Three membrane receptor proteins bind tightly to a hormone. The free hormone concentrations and their corresponding fractions of hormone binding, Y, are given in the table.

Cheat Sheet - Final Biochem Part IV

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