University:
Camosun College
Course:
BIOL 252 | Pathophysiology for Nursing 1
Academic year:

2024
Views:

37

Pages:

2
Author:

Aysha A.

Elimination of Hemoglobin. Aim:- To find out haemoglobin content in 100 ml of blood wing, Sahlis method, principle A known Volume of blood is mixed with N.10 HCL Acid. heating will be formed the colour of diluted acid hematin is compared with Standard colour and from the extent of the dilution necessary to match the colour. the quantity of haemoglobin is directly read from the graduated marking of tube!" Requirements: Fingertip blood, Sahlis Hemoglobinometer, Sahlis pipette. N. 10HCL, Distilled water, Stirring glass rod. Saklis hemoglobinameter Contains:- A rack with Standards Fixed in form of ground glass.- A specially graduated tube in both in gram and percentage Scale. A glass rod to Stix / Stir. A blood Pipette with 20 cumm Capacity Talquirts Gowers, Haldanes Colorimetric, Spectrophotometric Gasometric, Alkauni homatin method can also be made use of estimation. of haemoglobin Procedure- Place N/10 HCL in the diluting tube cup to the mark 10 on the percentage Scale. Obtain a large quantity of blood by finger puncture and drame the blood up to 2004 mark, taking necessary precautions to avoid air bubbles in blood. Columns to avoid off the excess in the pipette hipe dotting in blood from the pipette. Dip the pipi Pipette. Into the HCL. taken in the diluting tube and empty the blood into it. Drant up the Hel into the Pipette gently wash the blood into a diluting tube. This is repeated 2 or 3 times to rinse the pipette place the diluting tube between the Standards Such that the graduations Cannot be read as those Would interrupt Inlith the colour matching. Dilute the blood with distilled water till the colour of the solution. matches with the color of the Standard haemoglobin content can be read off directly in gms/100ml.