University:
Camosun College
Course:
BIOL 252 | Pathophysiology for Nursing 1
Academic year:

2024
Views:

266

Pages:

2
Author:

Aysha A.

Determination of Blood Group Aim: To find out the blood group to which an. individual belong to the principle:- Reaction between antigens and corresponding antibodies results in agglutination, - Requirements:- Three Clean glass Slides, 2 ml normal Baline in a test tube, Antiserum, A, B, D, 3 toothpicks, lancet for finger puncture. procedure:- A red cell, suspine of the blood to be tested, is prepared in a small clean and dry test tube. 1 ml of 0.9% Sodium Chlorine Solution is taken in test tube. - the finger is Sterilized with alcohol and using a 1. Sterile lancet the finger is prick deep 1-2 drop of blood are Sucked into a clean and dry pipette and put into the Saline in the tube. Mix well by gently rocking the tube." Three glass Slide are taken and placed on a whi Paper. Mark AntiA, AntiB, AntiD: one drop of Serum is placed on the corresponding Slide. A drop of red Cell Suspension is added to each Sera. Mix well using toothpicks. precaution should be taken to see that the Sergy (Inlithin 2 to 3 min. The slides are examined do not get contaminated. Agglutination Can be seen within 2 or 3 mina. The Slides aire examined with the naked eye first and then under the love pointer of the microscope to confirm. Agglutination. Sometimes the agglutination is delayed and appears at the end of 20 min. So the slides are examined again after 20 min. if there is agglutination With only AntiA Serum, the blood belongs to A blood group. if there is agglutination with only Anti-B serum. the blood belongs to AB blood group. if there is no agglutination with both Anti-A and Anti-B, the blood belongs to o blood group. if there is agglutination with anti sera The blood belongs to the RHtve Blood group. if there is agglutination with anti-D, the blood belongs to RH-Ve Blood group.