University:
Camosun College
Course:
BIOL 252 | Pathophysiology for Nursing 1
Academic year:

2024
Views:

35

Pages:

3
Author:

Aysha A.

Study of Hemocytometer Aim: To understand the design of a hemocytometer, an instrument often used to count blood cells. Principle: The hemocytometer is constructed by dividing a square of 3 mm into several smaller scades squares on a high-quality, Specially thick glass slide. The volume of diluted blood in any Square or Square Can arrive 0.1 and Cell counted can be related to each Cumim of blood Requirements:- Hemocytometer with Cover glass.. "The Hemocytometer Set consists of :- The counting Chamber (Neubauer). The blood pipette:- one for diluting the blood for RBC count One for diluting the blood for WBC count Hemocytometers are of several types. A fene examples are as follows:- Neubauer Double counting Chamber. Thoma Counting Chamber. I 3. Burkee counting Chamber. Fuck's - Rosent hall. Spiers levy "They deffer from each other in the Way of Squares marked.20 The Neubauer Hemocytomet it has twee vertical moast or It is a thick glass slide depressions on either fide. Enclosed within this area is another H-Shaped moat, the horizontal limb of which separates the area on which the squares are marked. Thus, we have Auto Squares on the two sides of the horizontal limb of the H. the Surface on which the Squares are marked is exactly o.1 mm below the general Surrfare of the slide so that when a cover glass is placed Over the film of blood. the film will have a thicknes Of 0.1 mm. Blood Pipette:- RBC Pipette:-It has a capillary Stem of a Uniform bore with a Well ground / ground conical tip just above the stem is The bulb capacity of Stem is one and that of the bulb is 100 times that is the Stem. the stem is divided into lo equal parts and marked 0.5 and 1. just above the bulb is the mark 101. The bulb contains a red bead with help. mixing blood with diluting third For identification of pipette To find the bulb Other weise it will stick to the side WWBC pipette: It has a stem with a lide and a capillary bore. it has also 10 divisions and is marked 0.5 and 1. the bulb is smaller and has a white bead. procedure:- Clean the counting chamber and place it on the Stage of the microscope. Focus lone pouler objective first and observe the general Structure of the dines close donen the diaphragms of the microscope to minimum aparture the lines Inwill become clear. The counting Chamber is a Square of 5 mm it is divided into 9 equal squares by triple lines so that each square has an area of 1 sq.mm. The squares in the four corners have different rulings/rutings from the Central Square. The comer squares are used for counting RBC- The central Roe Square is divided into 25 large Squares by triple lines. The middle of the 3 lines from the margin of each square. each of these 25 large Squares is again divided into 16 small squares by single lines so that each small squares is edge 1/20mm at the