University:
Camosun College
Course:
BIOL 252 | Pathophysiology for Nursing 1
Academic year:

2024
Views:

284

Pages:

2
Author:

Aysha A.

Page No.DateScheme for iden tification of unknown Carbohydrates *Un Known SolutionMolisch's test (1 ml alpha naphthol + 1 m Cone Hbsory).No change (proteins or lipids)Reddish voilet Ring (carbohydrataIodine test (12ml of Solution2 or3 drops iodine Solution)Blue (may be Starch)Red (may be dex (trin). Nocolor.Benedict's test(may be mono orAfter Hydrolysis.Benedict 3 testdisaccharide).( 1 ml sucrose +5 drop cone Hat ( light green color)2 min boilt 5 drop 40% NaoH,)(5ml benedict'sBenedict'streagent + sdrops( ml benedict'sStarchsugart 2min boil.).reagent +8 dropssugar rt2minboil)Benedict's testAfter hydrolysis.(1ml of surrose + 5 drops cone Red precipitate No.Hel + 2min boil + 5 drops(reducing precipitate40% NaoH ).Sugar).Dextrinmay be SucroseBarfoed's test(2ml barfoed's reagent +Benedict's test after hydrolysis2ml Sugar solution + Keep( 1m surrose drop cone.Hu +2min in boiling water bath: +2min boil).Phosphomolybolic and Page No.DateSeliwanoff's test Lied colorDeep bluelight blue( mono saccharidal)(disaccharides).(3ml Seliwarff's Yeagent1 ml Sugar solution + 30feOSazone testboil).Seliwanoff's test(red color).(15ml sugar solution( 3ml seliwanoft's10 drop glacial areticSucrosereagent + 1ml sugaracid + half Spatulasolution + 30 See boil).of phenyl hydrazinehydrochloride 1 Spatulaof sodium aletate clystals +Red color Nored color mix t warm, filter, KeepinFructose Glucoseboiling Water bath 20-30 min + cool.)SunflowerHedge hogcrystalcrystalMaltoselactoseY