University:
Camosun College
Course:
BIOL 252 | Pathophysiology for Nursing 1
Academic year:

2024
Views:

173

Pages:

2
Author:

Aysha A.

Total leucocyte count (White blood cell count). Aim:- To count the number of WBi's in 1 worm of blast, 1. Principles- counting known Wihite blood cells from knowing Volume of diluted blood and calculating the cells per curren of undiluted blood Requirments:- Fingertip blood, Haemocytometer, cover glass, stic pipette diluting fluid (Turk's Bastion), Microscope, lancet Cotton and Sprit composition of Turk's fluid:- Glacial arctic cuid-2nd-destroys red blood cells. Gentian Voilet- 0.25 gms - Stairi nucters of WeBe's Distilled water- 100 ml - an a Solient other diluting agents such as- 1 of HU 24. of acetic acid. procedures- pour a small quantity of Webe diluting fluis into the watch glass. prick the finger and draw blood upto 05 mark in the Wibe pipette. Draw diluting fluid upto Mark II, fautor The diluting & is 1:20, mix it by rolling the pipette on the Chamber, forms the field weith low power objective (lox) palm, Keeping it horizontally. Discard one or theo drops Estem doesn't have any blood cells) and Change the countin' Many of the -on the paper and enter the number in- Counting is to be done in each high power objective , counting the cells in all the four corners. Normal Values 4000 to 11000 / cumm of blood. Result: The given sample of blood hou. 6950 thousands. I cumm of blood