University:
Eastern Kentucky University
Course:
BIO 111 | Cell and Molecular Biology
Academic year:

2021
Views:

460

Pages:

3
Author:

Kristopher Elliott

Name: DNA Structure and Replication worksheet 1) If the sequence of the 5'-3' strand is AGTGCTAC, then the complementary sequence has the following sequence: (make sure to label ends) 2) On this picture… (if you don’t have touch screen capability / ability to draw on the sheet, if you can print you can do this as well. The main point? That you can accomplish each of these tasks!) a) Label the 5’ and 3’ ends of each strand of DNA, b) Circle and label ONE and only one phosphodiester bond, including all the atoms that are part of that bond. c) Put a P next to or on top of each purine nitrogenous base d) Put a Y next to or on top of each pyrimidine nitrogenous base e) Circle and label ONE deoxyribose sugar. f) Identify a GC (or CG) pair g) Identify an AT (or TA) pair 3) If DNA polymerase started adding on at the -OH on the DNA strand that is just above the “adding” in this sentence, and the new incoming nucleotide looked like this (below), could this nucleotide be added? Y/N Could another nucleotide be added after that? Why or why not? For this Q, assume there is much of the strand on the right that is just not shown and that it continues down and to the right on this page. Hint: read the bit in your textbook about DNA sequencing. 4) If this is your replication fork: FYI: B and D refer to the whole “strand”, whereas the other letters are more specific. Identify: A B C D E F G H 5) If the template strand on the bottom read “GGCCATAGGAT” in a segment where a new RNA primer needs to be synthesized (marked with yellow in picture above), what would be the primer sequence? (don’t forget to label the 5’ and 3’ ends of your primer). Template: GGCCATAGGAT Primer: 6) What was the directionality (which is 5’ end, which is 3’ end of the template for Q4)? _____GGCCATAGGAT______ 7) If the black dots in the figure below represent the ori sequence, then, choosing from the letters shown, which two primers would be the first ones made as replication began at this point?