Gel Electrophoresis Read the following statements. Rewrite them so that they are correct if necessary. Use the Genetics – Molecular chapter of the lab manual to help you. 1) Each band in a DNA electrophoresis gel is made up of one molecule of DNA. Each band in a DNA electrophoresis gel is made up of billions of DNA molecules. 2) Gel electrophoresis can tell you the sequence of a particular DNA fragment. Gel electrophoresis is a technique used to separate DNA fragments according to their size. 3) You can see DNA on a gel because DNA is naturally fluorescent. A fluorescent dye has been added to the DNA so you can visualize the DNA bands. 4) DNA moves through a gel because it is positively charged and is attracted to the negative electrode. DNA molecules are negatively charged and will respond to an electric field and migrate toward the positive electrode. 5) The speed at which DNA moves through a gel is directly related to its charge. In an electric field, different sized fragments move at different rates. 6) An electrophoresis gel used for DNA is usually made from gelatin which is a protein obtained from seaweed. The gel is made from agarose, a substance found in seaweed, and its electrically neutral. 7) When visualizing your gel, you can tell the size of the DNA fragments by seeing how wide each band is. When visualizing your gel, you can tell the size of the DNA fragments by seeing how long each band is. 8) A gel is placed in a liquid called running buffer because it is an insulator and will protect the user from electric shock. A buffer solution is poured over the gel, so that the entire surface; Ions in the buffer solution conduct electricity during electrophoresis.