Simulating Filtration

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 4: Simulating Filtration Name: Date: 15 September 2020 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 Filtration is a process that You correctly answered: is passive. 2 Filtration is dependent upon a You correctly answered: hydrostatic pressure gradient. 3 The filtrate You correctly answered: All of these answers are correct. 4 An important place that filtration takes place in the body is in You correctly answered: the kidneys. Experiment Results Predict Questions 1 Predict Question 1: What effect will increasing the pore size of the filter have on the filtration rate? Your answer: The filtration rate will increase. 2 Predict Question 2: What will happen if you increase the pressure above the beaker (the driving pressure)? Your answer: The filtration rate will increase. Stop & Think Questions 1 The reason none of the solutes were present in the filtrate was that You correctly answered: the solutes were all too large to pass through. 2 What does an increase in the driving pressure correspond to in the body? You correctly answered: an increase in blood pressure. Experiment Data Run Number Solute MWCO Pressure (mm Hg) Filter Rate (ml/sec) Residue Start Conc. (mg/ml) Filter Conc. (mg/ml) 1 Na+ Cl- 20 50 1.00 present 5 0.00 1 Urea 20 50 1.00 present 5 0.00 1 Glucose 20 50 1.00 present 5 0.00 1 Powdered Charcoal 20 50 1.00 present 5 0.00 2 + 50 50 2.50 present 5 4.81 - Na Cl 2 Urea 50 50 2.50 present 5 0.00 2 Glucose 50 50 2.50 present 5 0.00 2 Powdered Charcoal 50 50 2.50 present 5 0.00 3 Na+ Cl- 200 50 10.00 present 5 4.81 3 Urea 200 50 10.00 present 5 4.74 3 Glucose 200 50 10.00 present 5 4.39 3 Powdered Charcoal 200 50 10.00 present 5 0.00 4 Na+ Cl- 200 100 20.00 present 5 4.81 4 Urea 200 100 20.00 present 5 4.74 4 Glucose 200 100 20.00 present 5 4.39 4 Powdered Charcoal 200 100 20.00 present 5 0.00 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 After filtration, substances that pass through the filter are called the filtrate, which includes You correctly answered: all of the above. 2 The top beaker in the simulation corresponds to You correctly answered: the blood capillary. 3 Why was there not 100% recovery of the Na+Cl- solute with any of the membranes? You correctly answered: Some of the solute remained on the membrane filter. 4 An increase in blood pressure would probably initially __________ filtration in the kidneys. You correctly answered: increase the rate of. Review Sheet Results 1 Explain in your own words why increasing the pore size increased the filtration rate. Use an analogy to support your statement. How well did the results compare with your prediction? Your answer: Solutions will run through a filter membrane faster if you increase the size of the pore, because it the larger opening creates space for more movement. 2 Which solute did not appear in the filtrate using any of the membranes? Explain why. Your answer: Powdered charcoal did not show up on any filtrate, due to the fact that it's size is smaller than any of the pores used. 3 Why did increasing the pressure increase the filtration rate but not the concentration of solutes? How well did the results compare with your prediction? Your answer: Increasing the pressure above the beaker will only result in the solution running faster, while the solute concentration stays the same. Solute concentrations are not affected by pressure.