Blood Typing Lab

5/17/2020 PhysioEx Exercise 11 Activity 4 PhysioEx Lab Report Exercise 11: Blood Analysis Activity 4: Blood Typing Name: Date: 17 May 2020 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 Red blood cell membranes have You correctly answered: agglutinogens that specify that individual's blood type. 2 ABO and Rh antigens You correctly answered: All of these answers are correct. 3 Which of the following statements about blood types is correct? You correctly answered: A person with type O blood has two recessive alleles and has neither the type A nor type B antigen. 4 Antibodies to the A and B antigens are You correctly answered: found preformed in the blood plasma. Experiment Results Predict Question 1 Predict Question: If the patient's blood type is AB- , what would be the appearance of the A, B, and Rh samples? Your answer: A, clumped; B, unclumped; Rh, clumped. Stop & Think Question 1 Why are individuals with the AB+ blood type known as universal recipients for blood transfusions? You correctly answered: They have both A and B antigens on the surface of their RBCs, and their blood serum does not contain antibodies against A, B, or Rh antigens. Experiment Data Blood sample Agglutination with Anti-A Serum Agglutination with Anti-B Serum Agglutination with Anti-Rh Serum Blood Type Rh factor 1/4 5/17/2020 PhysioEx Exercise 11 Activity 4 1 positive negative positive A + 2 negative positive positive B + 3 positive positive negative AB - 4 negative negative negative O - 5 positive positive positive AB + 6 negative positive negative B - Sample 6 2/4 5/17/2020 PhysioEx Exercise 11 Activity 4 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 To determine an individual's blood type, drops of the blood sample are mixed You correctly answered: separately with antiserum containing antibodies that recognize either type A antigens, type B antigens, or Rh antigens. 2 Agglutination You correctly answered: All of these answers are correct. 3 Antigens You correctly answered: are present on all cells. 4 ABO and Rh agglutinogens You correctly answered: cause the most vigorous and potentially fatal transfusion reactions. Review Sheet Results 1 How did the appearance of the A, B, and Rh samples for the patient with AB- blood type compare with your prediction? Your answer: My prediction did not match the results because A was clumped, B was clumped, and Rh was not clumped, it was smooth. 2 Which blood sample contained the rarest blood type? Your answer: AB- contained the rarest blood type. 3 Which blood sample contained the universal donor? Your answer: O- contained the universal donor. 3/4 5/17/2020 PhysioEx Exercise 11 Activity 4 4 Which blood sample contained the universal recipient? Your answer: AB+ contained the universal recipient. 5 Which blood sample did not agglutinate with any of the antibodies tested? Why? Your answer: Sample 4 didn't agglutinate with any of the antibodies tested because none of the antigens were present. 6 What antibodies would be found in the plasma of blood sample 1? Your answer: Anti-B antibody was found in sample 1. 7 When transfusing an individual with blood that is compatible but not the same type, it is important to separate packed cells from the plasma and administer only the packed cells. Why do you think this is done? (Hint: think about what is in plasma versus what is on RBCs.) Your answer: This is done because the plasma contains antibodies that can react with the recipients antigens on red blood cells. 8 List the blood samples in this activity that represent people who could donate blood to a person with type B+ blood. Your answer: B+ (sample 2), B- (sample 6), O- (sample 4). 4/4