University:
Eastern Kentucky University
Course:
BIO 315 | Genetics
Academic year:

2022
Views:

350

Pages:

2
Author:

Hallie Randolph

How is the lac operon regulated? Transcription of the lac operon results in the production of how many diﬀerent mRNA transcripts? The absence of lactose results in the lac repressor protein _________________. Which gene is responsible for producing the protein that converts lactose to allolactose? If a cell has a mutation in the lacY gene that produces a non-functional product, how is regulation of the lac operon aﬀected? An inducer binds to an activator protein and inhibits transcription. The lacI gene makes a repressor protein The structural proteins encoded by the lac operon are involved in lactose synthesis The CAP protein is an activator that binds to the operator The product of lacI is an allosteric protein that can undergo a conformational change when allolactose binds to it CAP binding causes the DNA to bend facilitating RNA polymerase binding Which of the following sets of conditions would result in high levels of gene transcription from the lac operon? How many binding sites are there in the lac operon for the lac repressor? Not all of the operator sites in the lac operon have the same eﬀect. Consider the data below. The combination of which two operator sites result in the lowest amount of repression? The cAMPÐCAP binding site is between operators O1 and O3. When cAMP-CAP is bound it facilitates binding of the repressor. Under what conditions would this occur? The lac repressor binds to what site within the lac operon? Which of the following is not part of the lac operon? The lac repressor is inactivated by binding to which of the following? Polycistronic mRNA is transcribed from the lac operon when lactose is present in the cell. The lac repressor protein is active in the absence of lactose within the cell. When glucose is present, __________. The lac repressor __________. When both glucose and lactose are present __________. In the absence of glucose and presence of lactose, allolactose combines with the repressor and allows RNA polymerase to proceed with transcription. When both glucose and lactose are absent, transcription of the lac operon occurs. How would the interpretation of the Jacob, Monod, and Pardee experimental results change if lacO was mutated instead of lacI? Attenuation of the trp operon requires which conditions to be met? You identify a strain of bacteria that has a mutated trp operator that cannot bind the repressor. Under what circumstances will the trp operon be transcribed? Which of the following would not be considered a reversible posttranslational modiÞcation? Translation of mRNA transcribed from the trp operon can be regulated by formation of a secondary structure within the mRNA. The mRNA that forms this secondary structure is transcribed from which gene in the trp operon?