7.014 Quiz II (Solutions) - Edubirdie

University:
Massachusetts Institute of Technology
Course:
7.014 | Introductory Biology
Academic year:

2022
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416

Pages:

4
Author:

limeja9tu

7.014 Quiz II **This will be a closed book exam** Solutions: Question 1 a) i) template 1 template 2 template 3 template 4 ii) B and C iii) The lagging strand is more affected by the lack of DNA ligase. DNA replication on the lagging strand occurs in small stretches called Okasaki fragments. For replication of the lagging strand to be complete, a phosphodiester bond must be formed between the 3'OH on one Okasaki fragment and the 5' phosphate on the other. DNA ligase makes this bond. b) Growing DNA chain CH 2 O C CH3 dCTP O -O C N C C OH H N O O P P -O O c) Enzyme Activity Function(s) Topoiosmerase k Primase (synthesizes primer) e, f, j DNA polymerase to elongate new DNA strand b, c Helicase to unwind DNA h DNA polymerase to replace RNA with DNA b, d Processivity factor i Question 2 a) 5’ G A A U C G C U A C A A 3’ O -O NH 2 O O P -O N O CH 2 O C C C C OH H N O

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