Virtual Microscope Activity

Virtual Microscope Part A: Learn-Click the "Learn" tab on the bottom of the simulation to learn more about the microscope. Click each question mark you see in the simulation As you click on each of the microscope parts, read the pop-up to answer the following. 1. Identify each of the following by letter. K On/Off Switch Eyepiece/Ocular Lens Arm F Nosepiece G Objective Lenses Stage H Diaphragm E Stage Adjustment M Base L Coarse Adjustment Lens Paper N Fine Adjustment B Immersion Oil p Slide box Kimwipes 0 0 F G B H (N) 0 P 2. Explain what each of the following does: kimwipes Kimuipes are used to clean laboratory equipment and instruments, fibre opties connectors, camera lenses. Not for microscope lens immersion oil A special of used with OOX or leger kins, a drople placed on a cover between the glass lone and glass side which narsa reclution diaphragm of the moge Most sear at higher powers, the diaplinam adjusts the amount of light possing frough frough the state oporning ft holes to iluminate the specimen while sc stage adjustment knob nerostiny contrast and relation The mecharvical stage allous for adjustment for the slide position forward, baciousand and left or right Part B: Magnification - Click the "Guide" tab on the bottom of the simulation to learn more about the objective lenses. Choose "Objective Lenses" read the information and answer the following questions: 1. What is the most common magnification of the eye piece lens? IOX 2. Objective lenses come in a range of magnifications. What are the 4 commonly used lenses on microscopes? 4X (scanning) 10X (low power) 40X (High power) and 100X (oil immersion) 3. How do you calculate the magnification of a slide? Multiply the power of the eyepiece by the power of the objective lens. 10X X 40X400X 7 ↑ Eyepiece Objective Total magnification Part C: Sample Slides - Letter E-Go to the "EXPLORE" tab to view slides. You will need to use the coarse and fine focus and adjust the light to get clear views of the following. The slide can also be moved within the viewing field. 1. Include a screenshot of the letter "e" at 10x. To take a screenshot, use the print screen on your keyboard or use your phone to capture the screen. Insert the image in the space below. Your image should clearly show that you viewed the "e" and focused it. Screenshot does ruin the quality of the pic : 2. What did you need to do before viewing the slide at 100x? I needed to turn the nosepiese halfway, add immersion oil, then snap the lens into place. 3. A common mistake for beginners is thinking that the "e" just disappears at the high magnification. How would you explain to a younger student what happened to the e as you increase the magnification? What happens is the microscope gets aloser to the image making it look like it disappeared but it just got oloser to the object. For example, if you place your hande 60cm away from you eyes, you would see all of your hand, the lines, and the fingers. But if you put the hand 2 om away from your eye, you wouldn't see all of your hand and you wouldn't be able to see your fingers, that's exactly how the 100X makes the e disappear because it's so close that you wouldn't see the whole image anymore. Part D-Plant Cells Return to the slide box and choose plant slides plant cells. Focus with 4x, 10x, and 40x. You may need to adjust the light and center your slide. 4. Include a screenshot of the plant cells at 40x. 102 100% 5. Plant cells can be identified by their boxy appearance, where lines represent cell walls. Within the cell are green circles which are chloroplasts and a dark gray area which represents the nucleus. At 40x, how many individual cells can be seen in the viewing field? 10 Part E: Human Blood Return to the slide box and choose human blood Focus with 4x, 10x, and 40x. You may need to adjust the light and center your slide. 1. Include a screenshot of the blood cells at 40x 4X 100% 2. Blood cells can be identified by their round appearance. Unlike plant cells, they do not have cell walls. At 40x, how many individual blood cells can be seen in the viewing field? 134