Center de Collection de Typers Microbines (CCTM)

University:
The University Of Western Ontario
Course:
ANAT 2200B | Systemic Anatomy of the Human Body
Academic year:

2022
Views:

270

Pages:

3
Author:

Kyuwon J.

Center de Collection de Typers Microbines (CCTM) ● Collects, preserves ,authenticates and distributes ○ Types of strains ■ Souches types ○ Reference strains ■ Soches de reference ○ Clinically/industrially significant strains ■ Bacteria ■ fungi/easts ■ Rarely, viruses or parasite also depended on this center ○ Library of Microorganisms ○ Strains have verified identity ○ Stable storage condition ● Type strain ○ Souche type? ○ Reference of strain to a species ○ Used for standard naming and classifications ○ Species identification tough taxonomy ○ Essential on confirming isolated strains ■ Know it belongs to certain species ○ ex) ■ Lab isolate bacterius ⇒ hypothesize its Staphylococcus aureus and in comparison with validate reference strain, they were able to get help confirming its identity ● Core function of microbial types ○ Collection + documentations ■ Receiving strains from researchers, hospitals, and industry ■ Records full metadata ● Origin ○ Clinical ○ Environmental ○ Industrial ● Isolation date + condition ● Req on growth ● Safety levels ○ Biosafety classifications ○ Authentications ■ Verification of the microorganisms ● Phenotypic tests ○ Culture ○ Biochemical reactions ● Genotypic methods ○ PCR ○ Sequencing such as 16S ○ rRNA for bacteria ● Purity checks ○ Check for possible contaminations ○ Preservations ■ Long-term storage ■ Cryopreservation ● -80℃ liquid nitrogen ■ Lyophilization ● Freeze-drying ● Dessiccation ○ Distribution ■ Supplying strains to labs over globe ● Teaching labs ● Diagnostic validation ● Research experiments ● Pharmaceutical and biotechs Goal here is to maintain genetic stability and viability EX) ● Collection type refers to reliable, which would later be used as reference banks in case of microorganisms ● Type strain is the reference strain that will define this species ○ Reference strain would be used universally to identify once strain isolated ● CCTM support ○ Quality ○ Reproducibility ○ Taxonomy ○ Safety