Chapter V: RF Practicum ( Rheumatoid Factor)

CHAPTER V RF PRACTICUM (RHEUMATHOID FACTOR) BRIEF DESCRIPTION Factor rheumatoidis a macroglobulin in serumwith thatIgG hasinantibody properties to Immunoglobulin G (IgG). Apart from reacting human serum, rheumatoid factorwith can also reactIgG, withis usually IgG in rabbit serum. Rheumatoid factor, which only reacts human found in rheumatoid arthritis sufferers, but may also be presentsointhis non-rheumatoid sufferers and several other diseases, such as hepatitis, test is not specific. Therefore, tonamely detect rheumatoid factor, it is better to carry out two types of testing together, testing forWaaler the presence of anti-human IgG and testing for the presence of rabbit IgG (Rose test). Both types of testing can be carried out by passive agglutination by reacting the patient' sand serum withcarriers which wasvarious sensitized with IgG derived from human serum rabbit serum. As a carrier, particles can be used, but the preferred ones are latex and erythrocytes. PRINCIPLES OF EXAMINATION Antigens in thecoated form ofrheumatoid factoringlobulin serum reacts a suspension of latex particles with human gamma to formwith agglutination. PURPOSE OF THE EXAMINATION The to: expected competencies after doing this practicum are students will be able 1. Know and practice inspectionsRF (Rheumatoid Factor). 2.particles Be ablecoated to explain interpret serum reacting withagglutination. a suspension of latex withand human gamma globulin to form STUDY GUIDE To achieve competency in carrying out this practicum, students must first 1. Read and understand the contents of this learning module. 2. Explain and define inspection checksRF (Rheumatoid Factor). 3. Practice inspection and inspection inspectionRF (Rheumatoid Factor). 4. Make a results report after the practical lecture. PRACTICUM 5 RF(Rheumatoid Factor) - 150 Minutes EXAMINATION PROCEDURE A. Tools and Materials 1. RF latex 2. Positive control 3. Negative control 4. Usable test cards or slides 5.Drop stirreror Pipette and stirrer 6. Serums B. How to Check 1) Qualitative Test Procedure (Screening Test) a.serum Placesamples and warm the latex reagent, positive control, negative control and at room temperature. Gently homogenize the latex reagent before use. b. Pipette into a separate circle from the slide 1. Serum sample 1 drop 2. PC 1 drop 3. NC 1 drop c. LR, white cap, place into sample and control 1 drop each. d.areaMixofwith different stir sticks and distribute the liquid over the entire circle the slide. e.or Shake the slide slide on forthe 2 minutes to allow place the automatic rotatortheatmixture 100 rpm.to rotate slowly in a circle f. After 2 minutes read the results under bright light. g. Description: 1 drop (40 µl/ 50 µl). 2) Semi Quantitative Test Procedure a. The procedure is the same as qualitative. b. Dilution is carried out as below: C. Interpretation of Results •conversion Read the titer on (looking the last dilution with agglutinationlook and multiply by the factor at the reagent sensitivity value) to get the result in the IU/L; for example, the final dilution of agglutination is obtained at 1:16, then RF titer is 16 X 8 [IU/L] = 128 [IU/L]. EXERCISE 1.IgG,Disease(Rheumatoid of an antibody response producing how long can IgG beFactor)is detectedtheinresult the body? a. 7 days b. 14 days c. 60 days d. 3 days e. 40 days 2.after Ancarrying ATLM obtained a blood sample from a patient withwasrheumatoid symptoms out an examination but no agglutination found. so what action do you take? a. Re-examination (duplo) was carried out. b. Observe under a microscope so that the agglutination is clearer. c. The results are carried out as they are d. Check the patient's history e. First, see whether the antigen and control are still good. 3.comorbidities The RF test can non-specific reactions if the patient has thatproduce can cause? a. Anemia b. Cardiovascular c. SLE d. Tuberculosis e. Autoimmune SUMMARY •toRheumatoid factor is a macroglobulin in serum that has antibody properties IgG. •sufferers Rheumatoid arthritisother sufferers, but itsuch mayasalso be present in test non-rheumatoid and several diseases, hepatitis, so this isserum not specific so the RF test is needed for further diagnosis by seeing the react a suspension of latex particles coated with human gamma globulin to formwithagglutination. GLOSSARY 1. Carrier, that is, the carrier of the disease can be passed on to offspring. 2.reaction Immunoglobulin is aantibodies. globulin protein resulting from an immune response that produces