University:
University of Cincinnati Blue Ash
Course:
BIOL 5180 | Immunology
Academic year:

2024
Views:

59

Pages:

3
Author:

Jasper J.

COMPATIBLE CROSS TEST EXAMINATION Method : Tube / Conventional Preparation of tools, reagents and examination materials: 1. Turn on the incubator/water bath at 37ºC 2. Leave the Reagent at room temperature before use 3. Store the reagent at 2ºC-6ºC after use 4. The blood to be examined must be blood without anticoagulants or blood with anticoagulants that must not be older than 48 hours. 5. Separate the serum/plasma from the red blood cells 6. Wash the red blood cells to make a red blood cell suspension Reagents: 1. Saline / NaCl 0.9% 2. Bovine Albumin 22% 3. Coombs Serum 4. Coombs Control Cell Equipment : 1. Test tube measuring 12 x 75 mm 2. Incubator / water bath 37ºC 3. Centrifuge 4. Object Glass 5. Microscope 6. Pasteur Pipette 7. Spray Pumpkin 8. Tube rack MATCHED CROSS TEST FOR 1 DONOR Phase I: Room temperature phase in saline medium 1. Take 3 tubes, insert them into each tube: ● Tube I Major test : 2 drops of patient's serum and add 1 drops of donor cell suspension 5% ● Tube II Minor tests: 2 drops of donor plasma and add 1 patient cell suspension drops 5% ● Tube III Auto control : 2 drops of patient's serum and add 1patient cell suspension drops 5% 2. Shake the contents of the tube until homogeneous, then rotate at 3000 rpm for 15" 3. Read the reaction to hemolysis and/or agglutination macroscopically 4. Reading the results: No hemolysis and/or agglutination occurred. : proceed to phase II Hemolysis and/or agglutination occurred. : unsuitable/incompatible, Continue: ● In major: ABO & Rhesus blood group examination, antibody screening examination and antibody identification examination ● In minor/auto control: ABO & Rhesus blood group examination, matching cross test Phase II: Incubation phase at 37ºC in bovine albumin medium 1. Add to each tube 2 drops of 22% bovine albumin 2. Shake the contents of the tube and incubate at 37ºC for 15 minutes 3. Rotate at 3000 rpm for 15 seconds 4. Read the reaction to hemolysis and agglutination macroscopically 5. Reading Results: No hemolysis/agglutination occurred. :Proceed to phase III Hemolysis/agglutination occurred :not suitable/incompatible, continue ● In major: ABO & Rhesus blood group examination, antibody screening examination and antibody identification examination ● For minor/auto control: ABO & Rhesus blood group examination, Direct Coombs Test and eluate examination Phase III: Antiglobulin Test (AHG) Phase 1. Wash the red blood cells in the tube 3X with saline 2. Add to each tube 2 drops of Coombs serum 3. Shake the contents of the tube and rotate for 15 seconds 4. Read the reaction results macroscopically and microscopically 5. Reading results: No hemolysis/agglutination occurs : Suitable / Compatible (blood may be given to the patient) Hemolysis/agglutination occurs :not suitable/incompatible (blood should not be given to the patient), continue: ● In major: Antibody screening examination and antibody identification examination ● In minor/auto control: Direct Coombs Test and Eluate inspection All Coombs Test results were negative, each tube: 1. Add 1 drop of Coombs Control Cell (CCC) 2. Shake the tube until mixed 3. Rotate at 3000 rpm for 15 seconds 4. Reading results: Agglutination : Inspection results are correct No Agglutination: The examination results need to be repeated