Validation Examination of ABO Blood Group Reagent

VALIDATION EXAMINATION OF ABO BLOOD GROUP REAGENTIA,RHESUS AND CROSS TESTS ARE COMPATIBLE Objective : To find out/determine the reagents that will be usedused as valid or invalid Method : Tube Reagents : 1. Anti A 2. Anti B 3. Anti D 4. Cell A Test 5. B Cell Test 6. O Cell Test 7. Bovine Albumin 22% 8. Coombs Serum 9. Coombs Control Cell Equipment : 1. Centrifuge 2. Pumpkin Spray 3. Reaction Tubes 4. Tube Rack Ways of working : 1. Anti A 1. Prepare 3 tubes on a shelf, label them I, II, III 2. Fill each tube with: ● Tube 1 : 1 drop of A cell test 5% ● Tube II : 1 drop of B cell test 5% ● Tube III : 1 drop of O cell test 5% 3. Place 2 drops of anti-A each in tubes I, II, III 4. Shake all the tubes until mixed 5. Rotate 3000 rpm for 15 seconds 6. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I agglutination occurs (positive) ● In tube II no agglutination occurs (negative) ● In tube III no agglutination occurred (negative) 2. Anti B 1. Prepare 3 tubes on a shelf, label them I, II, III 2. Fill each tube with: ● Tube 1 : 1 drop of A cell test 5% ● Tube II : 1 drop of B cell test 5% ● Tube III : 1 drop of O cell test 5% 3. Drop 2 drops each of anti B in tubes I, II, III 4. Shake all the tubes until mixed 5. Rotate 3000 rpm for 15 seconds 6. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I no agglutination occurs (negative) ● In tube II agglutination occurs (positive) ● In tube III no agglutination occurred (negative) 3. Anti D 1. Prepare 2 tubes on a shelf, label them I and II 2. Fill each tube with: ● Tube 1 : 1 drop of 5% Rhesus positive O cell test ● Tube II : 1 drop of 5% Rhesus negative O cell test 3. Drop 2 drops each of anti-D in tubes I and II 4. Shake all the tubes until mixed 5. Rotate 3000 rpm for 15 seconds 6. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I : agglutination occurs (positive) ● In tube II : no agglutination occurs (negative) 4. Standard A Cell Test 1. Prepare 2 tubes on a shelf, label them I and II 2. Fill each tube with: ● Tube 1 : 2 drops Anti A ● Tube II : 2 drops Anti B 3. Place 1 drop each of 5% Standard A cell test in tubes I and II 4. Shake all the tubes until mixed 5. Rotate 3000 rpm for 15 seconds 6. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I : agglutination occurs (positive) ● In tube II : no agglutination occurs (negative) 5. Standard B Cell Test 1. Prepare 2 tubes on a shelf, label them I and II 2. Fill each tube with: ● Tube 1 : 2 drops Anti A ● Tube II : 2 drops Anti B 3. Place 1 drop each of 5% Standard B cell test in tubes I and II 4. Shake all the tubes until mixed 5. Rotate 3000 rpm for 15 seconds 6. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I no agglutination occurs (negative) ● In tube II agglutination occurs (positive) 6. Standard O Cell Test 1. Prepare 2 tubes on a shelf, label them I and II 2. Fill each tube with: ● Tube 1 : 2 drops Anti A ● Tube II : 2 drops Anti B 3. Place 1 drop each of 5% Standard O cell test in tubes I and II 4. Shake all the tubes until mixed 5. Rotate 3000 rpm for 15 seconds 6. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I no agglutination occurs (negative) ● In tube II no agglutination occurs (negative) 7. Coombs Serum (AHG) 1. Prepare 4 tubes on a rack, label them I, II, III and IV 2. Fill each tube with: ● Tube 1 : 1 drop CCC ● Tube II : 1 drop of Cell A Test 5% ● Tube III : 1 drop of Cell B Test 5% ● IV tube : 1 drop of Cell O Test 5% 3. Wash all four tubes with saline 1 x 4. Place 2 drops of AHG each in tubes I, II, III and IV 5. Shake all the tubes until mixed 6. Spin at 3000 rpm for 15 seconds 7. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I agglutination occurs (positive) ● In tube II no agglutination occurs (negative) ● In tube III no agglutination occurs (negative) ● In the IV tube no agglutination occurs (negative) 8. Bovine Albumin 22% 1. Prepare 2 tubes on a shelf, label them I and II 2. Fill each tube with: ● Tube 1 : 2 drops of AHG ● Tube II : 2 drops of Saline 3. Place 1 drop of CCC each in tubes I and II 4. Shake all the tubes until mixed 5. Rotate 3000 rpm for 15 seconds 6. Read reactions Reading of Results Read the reaction by shaking the tube gently ● In tube I agglutination occurs (positive) ● In tube II no agglutination occurs (negative)