Special Media for Isolating Bacteria Analysis

University:
University of South Florida
Course:
MCB 3020 | General Microbiology
Academic year:

2022
Views:

15

Pages:

3
Author:

Nesbyenvkk

Special Media for Isolating Bacteria Analysis 1) This table represents the composition of MSA and MAC plates compared to plain nutrient agar. Using the information from the videos and the Lab 4 introduction, highlight for Mannitol salt agar and for MacConkey agar the selective ingredient in green and the differential ingredient in yellow, some ingredients are just there to support growth and will not be either. For 1 liter of media Nutrient Agar Mannitol Salt Agar MacConkey Agar Peptone 20 gr 20 gr 20 gr NaCl 5 gr 75gr 5 gr 15 gr 15 gr Bile Salts Agar Mannitol 5 gr Lactose Phenol Red 15 gr 10 gr 10 gr 0.025 gr Neutral Red 0.03 gr Crystal Violet 0.001 gr 2) These are your plate results obtained following the direction in Lab 4 introduction and method: Complete the Table below using the above results: Organism Mannitol Salt Agar MacConkey Agar Growth Yes or No Color Growth Yes or No Color Escherichia coli no yes pink Staphylococcus aureus Experimental sample yes no color was developed yellow yes pink yes no pink 3) Answer the following questions: - Did you get the results that you expected for your control plates? (yes or no) E. coli MacConkey plate ____yes______ E. coli MSA plate ____no______ S. aureus MacConkey plate _____no_____ S. aureus MSA plate __yes________ - Did you get mannitol fermentation in your experimental sample MSA plate? (yes or no)______yes_______ - Did you get lactose fermentation in your experimental sample Mac plate? (yes or no) _________yes____ - Using the information from Lab 4 differential and selective media introduction, give the name (s) of 2 microorganisms that could be present in your experimental sample. ___________________S.aureus-mannitol salt agar______________  and ____________________________E.coli-_MacConkey plate______ 4) Many different media are used to differentiate and select bacteria. Blood Agar plates are often used in microbiology diagnostic as they allow hemolytic reactions to be observed on plates. Explore the sheep blood agar plate  and answer the following questions  A. Sheep blood agar plates are differential, selective or both? sheep blood agar is differential and enriched. B. What kind of hemolysis is depicted in the figure below? ___beta___________________________ C. Give the name of a bacteria that could give similar results ___alpha_________________________