PRACTICUM I DIFFUSION, OSMOSIS AND IMBIBISI A. Objectives To study the processes of imbibition, osmosis and diffusion of a substance. B. Introduction The process of water movement in plant cells can take place by diffusion, imbibition and osmosis, which is passive transport. Diffusion is the movement of molecules or ions from one place to another due to random kinetic activity. Imbibition in plant cells is the absorption of water by forming compounds protoplasm and cell walls, especially macromolecular compounds such as proteins, polysaccharides, etc. The process includes diffusion and capillary movement.minute sub-microscopic capillaries). Water will the lower one. This event can only take place if the imbibant contains sub-microscopic gaps which function as pipes/capillary tubes (moving from areas that have high water potential to areas that have high water potential). Meanwhile, osmosis is the movement of water from a solution that has it high water potential to lower potential, through a differentially permeable membrane separate the two solutions. Differential permeable membranes have different permeability different for different compounds; can pass water molecules but inhibit the entry of dissolved compounds. C. DIFFUSION Tools and materials. 1. 4 test tubes 3. Methylene blue 1% 2. So that the market / so that it can be swallowed Ways of working 1. Make an agar solution with a concentration of 1%, 0.8%, 0.6% and 0.4% 2. Put 10 ml into a test tube. Mark the height of the surface so that after frozen. Next, add 2 ml of 1% methylene blue to each tube. 3. Close the mouth of the tube to prevent evaporation, then place it on a suitable surface average for 2 days. 4. Make the results of your observations in graphical form. D. OSMOSIS Tools and materials 1. 1 test tube 2. 5% CuSO4 solution 3. 1 potassium ferrocyanide crystal Ways of working 1. Pour 5 ml of 5% CuSO4 solution into a test tube 2. Carefully insert a potassium ferrocyanide crystal about the size of a kernel of corn into the tube. The reaction is placed in an upright position. 3. Observe the nature of the membrane that occurs E. EFFECT OF DISSOLVED SUBSTANCES CONCENTRATION ON IMBIBISI Tools and materials 1. 5 jam bottles / Erlenmeyer 2. Oven, scale 3. Soybean or corn seeds 50 grams 4. NaCI solution 4 M, 1 M and 0.5 M Ways of working 1. Dry corn or soybean seeds in a 60 ºC oven for 2 days. 2. Make a solution of 4 M, 1 M and 0.5 M NaCI, each ± 200 ml. The four solutions each have an osmotic pressure of -130, -72, -38 and -19 ATM. 3. Put the prepared corn / soybean seeds into 5 bottles dried 10 grams each. 4. Then put into the bottle 1 to 4 NaCI solutions 4 M, 2 M, 1 M and 0.4 M until the soybean seeds are submerged. The 5th bottle was filled with water as a control. 5. Weigh the seeds from the five bottles after 2 days. 6. Make the results of your observations in tabular form, with the number of grams of imbibed water per grams of seeds as the ordinate and osmotic pressure as the abscissa.