University:
Cornell University
Course:
BIOMG 2800 | Lectures in Genetics and Genomics
Academic year:

2023
Views:

241

Pages:

2
Author:

ityeg3co

DNA Technology NAME:________________________ 1. Name two situations when DNA fingerprints are useful. _________________________________ and _______________________________ 2. How does the DNA migrate from one end of the gel to the other? 3. What cuts up the DNA into tiny fragments? _____________________________________ Use the gels below to answer the following questions. Multiple Choice ____ ____ ____ ____ ____ ____ ____ ____ ____ 1. To produce genetically engineered bacteria that make a human protein, which of the following steps does a scientist have to take first? a. Insert the human gene for the protein into a plasmid. b. Extract the protein from the bacterial culture. c. Use a restriction enzyme to cut out the gene from human DNA. d. Transform bacteria with the recombinant plasmid. 2. During genetic modification, a. a prokaryote is changed into a eukaryote. b. a cell takes in DNA from another source. c. a cell’s own DNA is inserted into a plasmid. d. a cell is mutated. 3. Analyzing DNA by gel electrophoresis allows researchers to a. compare DNA samples from different sources. b. determine whether a particular allele of a gene is dominant or recessive. c. compare the phenotypes of different organisms. d. cut DNA with restriction enzymes. 4. One function of gel electrophoresis is to a. separate DNA fragments. b. cut DNA. c. recombine DNA. d. extract DNA. 5. Knowing the sequence of an organism’s DNA allows researchers to a. reproduce the organism. b. mutate the DNA. c. study specific genes. d. cut the DNA. 6. Which of the following includes all the others? a. plasmid b. genetically modified bacterium c. foreign gene d. recombinant DNA 7. On an electrophoresis gel, band B is closer to the positive end of the gel than is band A. Which of the following statements is true? a. Band B is more negatively charged than band A. b. Band B moved faster than band A. c. Band A is smaller than band B. d. Band B consists of larger DNA fragments than does band A. 8. If two DNA samples showed an identical pattern and thickness of bands produced by gel electrophoresis, the samples contained a. the same amount of DNA. b. fragments of the same size. c. the same DNA molecules. d. all of the above 9. What is an advantage of using genetically engineered bacteria to produce human proteins? a. The human proteins produced by genetically engineered bacteria work better than those produced by humans. b. Genetically engineered bacteria can mass-produce pure human proteins. c. The human proteins produced by genetically engineered bacteria last longer than those produced by humans. d. Genetically engineered bacteria can produce human proteins used to make plastics.

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