University:
Liberty University
Course:
BIOM 500 | Introduction to Biomedical Sciences
Academic year:

2024
Views:

281

Pages:

6
Author:

Sheha A.

Electrophoresis It is a technique used for the separation of charged particles under the influence of an electric field. Principle: The movement of charged particles in an electric field resulting in their migration toward the oppositely charged electrode. The rate of migration depends on the net charge, size, and shape of the particle. many important biomolecules like amino acids, proteins, nucleic acids and nucleoides have ionizable groups and can exist as cations or anions depending on the pH of the medium. The electric field is removed before the particles reach the electrodes. A supporting medium is required to carry out the electrophoresis. It may be filter paper (paper electrophoresis) or glass slide coated with agar agarose gel electrophoresis Technique A small amount of sample is applied on the supporting medium near one margin The slide is kept on electrophoresis chamber and connected to the buffer compartment by filter paper strips. Electric field is applied - through electrode dipped in each buffer compartment This causes separation of sample into fractions Thus position is visible after staining with suitable dye The rate of migration of each component depends on the net charge, shape and size Electrophoretic chamber