Biuret Test - Edubirdie

University:
Liberty University
Course:
BIOM 500 | Introduction to Biomedical Sciences
Academic year:

2024
Views:

64

Pages:

5
Author:

Sheha A.

Biuret Test This test is a general test for proteins as it is considered by all proteins. Copper sulphate is added to proteins in the presence of NaOH. Observation: A violet color is obtained. Principle: Cupric ion in alkaline medium forms a violet colored complex with peptide bond nitrogen of proteins. The minimum requirement for the positive test is the presence of 2 peptide bonds in the molecule. Biuret test is specific for CO-NH bonds. Negative Biuret Test Positive Biuret Test Ninhydrin Test It is a general test for the detection of compounds having free amino groups. It is most sensitive test for amino acids. Observation: A violet color is obtained. Biuret Test Freshly prepared ninhydrin solution is added to amino acids/protein solution. Observation: A violet color is obtained.

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