University:
Liberty University
Course:
BIOM 500 | Introduction to Biomedical Sciences
Academic year:

2024
Views:

178

Pages:

7
Author:

Sheha A.

Properties of Isoenzymes Alternative form of some enzyme. Activity of isoenzyme can be different in different tissues. e.g., Salivary amylase and pancreatic amylase both are isoenzyme and are having different activity in different tissues. They are having different amino acid sequences. They are having the different amino acid composition. They have different in their physical and chemical properties which include the structural, electrophoretic and immunological properties. Diagnostic significance of enzymes As almost every biochemical reaction is catalyzed by the particular type of enzyme and alteration in the activity leads to under production of products from a particular or substrate. Thus, the measurement of certain enzymes in serum has been found to be helpful in the diagnosis of various disease because some disease due to alteration in functioning of enzyme. Serum Enzyme in Heart Disease When electrocardiogram (ECG) is difficult to interpret, the enzyme assays are carried out for the diagnosis of myocardial infarction. The enzyme for diagnosis of myocardial infarction: Serum Creatine Phosphokinase (CPK) / Creatine Kinase (CK) It is richly found in heart and skeletal muscle. The normal value varies from 10-15 IU/L. The activity of this enzyme is found to be elevated in all types of muscular dystrophies. Aspartate Transaminase Lactate Dehydrogenase Cardiac Troponin Serum CPK-3 activity also increases in patients with acute cerebrovascular disease whereas CPK-2 in plasma is virtually specific for infarction in myocardial.