University:
Liberty University
Course:
BIOM 500 | Introduction to Biomedical Sciences
Academic year:

2024
Views:

357

Pages:

5
Author:

Sheha A.

Electrophoresis It is a technique used for the separation of charged particles under the influence of electric field. Principle: The movement of charged particles in an electric field, separating charged particles according to their size. Electrophoresis: The rate of migration depends on the charge, size and shape of the molecules. Many important biomolecules like amino acids, proteins, nucleic acids, carbohydrates can be separated. Charged particles can exist as cations or anions depending on the pH of the medium. An electric field is applied between the electrodes. The particles migrate towards the electrodes depending on the charge. In electrophoresis, the medium where the separation occurs is called electrophoresis medium. The medium can be filter paper (paper electrophoresis) or gel (gel electrophoresis) coated with buffer. Technique: A small amount of sample is applied on the supporting medium. The supporting medium is one of its side is kept in an electrophoresis tank. The electrodes are connected to the power supply. A buffer solution is applied to the medium. When current is passed through the electrodes, separation of charged particles occurs. The rate of migration of each band depends on its charge, size and shape.