University:
Liberty University
Course:
BIOM 500 | Introduction to Biomedical Sciences
Academic year:

2024
Views:

377

Pages:

5
Author:

Sheha A.

**Test For Carbohydrates** **1. Molisch Test:** This test is a general test for the detection of carbohydrates. **Procedure:** 10ml of carbohydrate solution is taken. 3 drops of α-naphthol is added. Then 2ml of concentrated H2SO4 is poured along the sides of the test tube. **Observation:** A reddish violet ring is formed at the junction of 2 layers. **Principle:** Carbohydrates when concentrated H2SO4 dehydrate to form furfural or its derivatives. These condense with α-naphthol to form a colored complex. **1. Molisch Test:** * A reddish-violet ring is formed at the junction of the two layers. **Carbohydrates present:** * Carbohydrate is present. **2. Benedict's Test:** * This test is used to detect the presence of reducing sugars. * Principle: Reducing sugars contain free aldehyde or ketone groups. Carbohydrates containing free aldehyde or ketone groups reduce cupric ions (Cu2+) to cuprous oxide (Cu2O). Cupric ions (blue color) are reduced to cuprous oxide (brick red precipitate).

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