University:
Lindsey Wilson College
Course:
BIOL 1103 | Principles of Biology
Academic year:

2022
Views:

10

Pages:

2
Author:

Richard Mitchell

Lecture 13 Recombinant DNA nucleotide sequences from two diﬀerent sources, often two species, are combined in vitro into the same DNA molecule genetic engineering the direct manipulation of genes for practical purposes biotechnology the manipulation of organisms or their genetic components to make useful products DNA cloning Making identical multiple copies of a gene or other DNA sequence Plasmids are small circular DNA molecules that replicate separately from the bacterial chromosome Gene cloning involves using bacteria to make multiple copies of a gene restriction enzymes Bacterial cut DNA molecules at specific DNA sequences called restriction sites restriction fragments A restriction enzyme usually makes many cuts,yielding restriction fragments DNA ligase is an enzyme that seals the bonds between restriction fragments cloning vector In gene cloning, the original plasmid is called a cloning vector polymerase chain reaction PCR can produce many copies of a specific target segment of DNA gel electrophoresis One indirect method of rapidly analyzing and comparing genomes dideoxy chain termination method Relatively short DNA fragments can be sequenced by this Gene therapy is the alteration of an aﬄicted individual’s genes genetic profile can be obtained by analysis of tissue or body fluids GMP genetically modified products.