Cellular Respiration in Germinating Peas Activity 1 Data Table 1 Initial volume (Vi ) (mL) 25 Final volume (VF) (mL) 35 Total volume (VF–Vi ) (mL) 10 25 35 10 25 35 10 Sample Germinating peas Dormant peas + beads Beads only Activity 2 Data Table 2 Water temp. (°C) 21 22 Time (min) 0 5 Resp. #1 Volume in pipet Resp. #1 Change in volume Resp. #1 Corrected volume change Resp. #2 Volume in pipet Resp. #2 Change in volume Resp. #2 Corrected volume change Resp. #3 Volume in pipet Resp. #3 Change in volume .60 - - .61 - - .63 - .56 .04 .04 .61 .0 .0 .63 .0 22 10 21 15 22 20 .54 .02 .02 .59 .02 .02 .61 .01 .47 .07 .07 .58 .01 .01 .60 .01 .44 .03 .03 .57 .01 .01 .59 .01 Which of the respirometers serves as control? Explain your answer. • The respirator with the glass beads served as control because they are not alive, therefore they do not perform respiration. Assuming your experimental control measures worked, identify what variable(s) changed to bring about the observed change in volume (corrected change in volume in Table 1 and Table 2)? • The decrease in volume occurred due to the respiration of the peas. During cellular respiration, carbon dioxide is expelled, and oxygen is taken in. In this case, because potassium hydroxide (K0H) was used, some water was produced as a result of the mixture of potassium hydroxide and carbon dioxide. This resulted in a decrease in oxygen in the respirator, resulting in decreased volume. Summarize your results from your data tables. Compare the results from the respirometers containing germinating and dormant peas. Speculate about the cause(s) of any difference between the two pea samples, and explain your reasoning. Respirator one, which contained the germinated peas, had a higher respiration rate in room temperature water than respirator two with the dry peas because the peas are already beginning to grow. Because the germinating peas had the highest respiration rate, the biggest changes in volume were seen the longer the respirator sat in the room temperature water. Room temperature water is most effective in cellular respiration, therefore the colder the temperature, the slower cellular respiration occurs. The longer both respirators sat in room temperature water, the more the volume decreased due to lack of oxygen and the interaction between carbon dioxide and potassium hydroxide, thus exhibiting a successful experiment.

Cellular Respiration in Germinating Peas - Biology Lab Sheet

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