University:
Texas A&M University
Course:
BIOL 318 | Chordate Anatomy
Academic year:

2023
Views:

412

Pages:

33
Author:

Athena Harrison

LABORATORY EXERCISES FOR MALE REPRODUCTIVE SYSTEM Testis, rat. Nuclei of Sustentacular cells Leydig cells Spermatogonia Spermatocytes Spermatids sustentacular (“Sertoli”) cells pale nuclei of these cells are visible adjacent to the limiting membrane of the seminiferous tubules THE SERTOLI CELLS (SUSTENTACULAR CELLS) EXTEND THE FULL THICKNESS OF THE WALL OF THE SEMINIFEROUS TUBULE. IN OTHER WORDS, THE SERTOLI CELLS EXTEND FROM THE BASEMENT MEMBRANE TO THE LUMEN OF THE SEMINIFEROUS TUBULE. THE FUNCTION OF THE SERTOLI CELL IS SUPPORT FOR THE DEVELOPING GERM CELLS. Horse Sperm This EM at http://peer.tamu.edu/toolkit2.asp Slide #101 (1096). Testis, rat. Nuclei of sustentacular or Sertoli cells Myoid cell nucleus Spermatogonia seminiferous tubule lumen Spermatocytes Spermatids Spermatocyte Leydig cells Slide #101 (1096). Testis, rat. spermatids Meiotic figures (metaphase plates) Primary S. Secondary S. spermatid seminiferous tubule Myoid cell Spermatocytes Sustentacular cell nuclei or Sertoli cells Leydig cells WHEN A SPERMATOGOIUM CELL IS SIGNALED TO UNDERGO MEIOSIS INSTEAD OF MITOSIS, THE CELL HAS REPLICATED ITS Spermatogonia DNA TO BECOME 4N AND HAS ENTERED INTO THE FIRST PHASE OF MEIOSIS BUT NOT YET DIVIDED, SO THAT IT IS A DIPLOID 4N CELL, WHICH IS CALLED A PRIMARY Secondary spermatocytes (1°) SPERMATOCYTE. Meiosis Mitosis Meiotic Metaphase I base Mitotic metaphase Meiotic Metaphase II Mitotic metaphase Meiotic metaphase I Secondary spermatocytes lumen Human testis Epon toluidine blue stain 19680 SEMINIFEROUS TUBULES COMPOSED OF: SPERMATIDS SERTOLI CELLS MYOID CELL nucleus GERM CELLS SPERMATOGONIA, SPERMATOCYTES, SPERMATIDS Human testis Primary SPERMATOCYTES human Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat. seminiferous tubules rete testis and mediastinal testis Testis epididymis Leydig cells produce TESTOSTERONE interstitial tissue between the seminiferous tubules is quite thin. ductus deferens (tail of epididymis) Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat. CCT capsule’s proper TUNICA ALBUGINIA Leydig cells produce TESTOSTERONE The interstitial tissue between the seminiferous tubules is quite thin Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat. Sertoli cells; spermatogonia; primary spermatocytes; and early and late spermatids spermatogonia; Secondary spermatocytes Golgi phase Cap phase Elongation phase Maturation phase Testis, stallion. Sertoli cells; spermatogonia; primary spermatocytes; late spermatids Leydig cells sustentacular (“Sertoli”) cells; LEYDIG (INTERSTITIAL) CELLS ARE LARGE, ROUND TO OVAL CELLS WITH ACIDOPHILIC BUT OFTEN PALELY STAINING CYTOPLASM. SOMETIMES THESE CELLS CAN APPEAR FOAMY DUE TO THE FACT THAT THEY ARE STEROID PRODUCING CELLS. Testis, newborn kitten. Nuclei of sustentacular cells epididymis rete testis and mediastinal testis gonocytes (PM-172 b). Testis, epididymis, ductus deferens, pig (immature). rete testis and epidermidis Dividine gonocytes mediastinal testis gonocytes Nuclei of sustentacular (“Sertoli”) cells; (935). Testis, boar. DEGENERATION and loss of germ cells in the seminiferous tubules; however, it illustrates the open spaces between tall Sertoli cells where germ cells would have been located as indicated in the drawing of Sertoli cells Normal cat testis IF THE MALE IS A BILATERAL CRYPTORCHID THEN HE WILL BE INFERTILE; IF THE MALE IS A UNILATERAL CRYPTORCHID THEN HE likely WILL HAVE REDUCED FERTILITY. Cryptorchid testis Epididymis, goat. ductuli efferentes. ductuli aberrantes vestigial mesonephric tubules ductus epididymidis. Epididymis, boar. ductuli efferentes ductus epididymidis, Ductus deferens, stallion. thick tunica muscularis epithelium and lamina propria/submucosa smooth muscle Ampulla, donkey. proteinaceous material In lumen of the ampulla. CCT and smooth muscle. simple or stratified cuboidal epithelium Ampulla of ductus deferens, stallion. simple or stratified cuboidal epithelium Smooth muscle (Dog 1-160-1). Prostate, dog. simple cuboidal or simple columnar (like the prostate) and doesn’t have stereocilia. urethral lumen. TRANSITIONAL Prostate gland, dog. fibromuscular tissue Urethra with transitional epithelium small ampullae of the ductus deferens Prostate gland (pars disseminata), bull principal duct of the vesicular glands or ductus deferens; urethra SKELETAL MUSCLE, WHICH IS CALLED THE URETHRALIS MUSCLE OR THE EXTERNAL URETHRAL SPHINCTER Bulbourethral gland, boar. Vesicular gland, stallion. seminal vesicles because they are hollow, blind sacs vesicular glands or seminal vesicles Bulbourethral gland, goat. mucous character skeletal muscle fibers of the bulbospongiosus muscle. Penis, dog. pectiniform septum - separates the paired corpora cavernosum tunica albuginea (of the penis CCT) urethra -TRANSITIONAL PRIMARILY SMOOTH MUSCLE ( YOU MAY SEE A FEW SKELETAL MUSCL FIBERS MIXED IN WITH THE SMOOTH MUSCLE – ESPECIALLY PROXIMALLY dorsal penile artery, vein & nerveMUSCULAR ARTERY Penis, dog. Penis, dog. the glans penis and includes part of the prepuce internal lamina of the prepuce preputial cavity skin of the penis pars longa glandis - peripherally located erectile tissue urethra corpus spongiosum - erectile tissue surrounding the urethra os penis Penis, bull calf. corpus cavernosum penis, urethra, corpus spongiosum. skin of the penis and internal lamina of the prepuce are fused at this location Penis, boar (immature). corpus cavernosum penis, the urethra, and the corpus spongiosum. nerve, collagenous connective tissue (CCT), large vein, arterioles, venules, capillaries. nerve Small muscular artery, Unilocular adipose Tissue (white fat), Human Penis – transitional epithelium and surrounding spongy cavernous of penal urethra corpus cavernosum penis urethra nerve Transitional epithelium of urethra Corpus spongiosum skin Smooth muscle Tunica albuginea (of the penis, CCT) Corpus spongiosum

Laboratory Exercises for Male Reproductive System

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