University:
Texas A&M University
Course:
BIOL 319 | Integrated Human Anatomy and Physiology I
Academic year:

2021
Views:

103

Pages:

37
Author:

omiliar74be

Cell Structure III Cytoskeleton and Inclusions CYTOSKELETON MICROTUBULES (25 nM) MICROFILAMENT (6 nM) INTERMEDIATE FILAMENT (10 nM) EM 4: liquid droplets are sometimes clustered together in irregularly shaped groups visible in intestinal epithelial cells. Note: the large droplets visible in the goblet cell contain mucin, not lipid. 1. Lipid 2. Chylomicrons 3. Mucus EM 4 (Enlargement of section from class print): Microvilli at apical ends of intestinal absorptive cells with parallel arrays of microfilaments in their cores. The microvilli are covered with a wispy glycocalyx. The terminal web region immediately below the microvilli contains a very high concentration of microfilaments and (keratin) intermediate filaments, and thereby excludes most other cell organelles. 1. Microvilli 2. Glycocalyx 3. Terminal web EM 4b: intestinal absorptive cell (apex); 60,000x 1. Microfilaments 2. Microvilli 3. Mitochondria 4. Plasma membranes of adjacent cells 5. Terminal web 6. Zonula occludens EM 2b: Liver; cytoskeleton elements. Microtubules, microfilaments, and intermediate filaments can be compared in this cell, which has a high concentration of cortical microfilaments. 1. Microtubule 2. Microfilaments 3. Intermediate filaments EM 19c EM 6a: Insect spermatocyte. Centriolar region of a cell showing both the stable, triplet microtubule arrays within the centriole, and the labile, individual microtubules originating from pericentiolar material. 1. Centriole 2. Stable microtubule 3. Labile microtubule EM 6b: monkey oviduct; cross-sectional view of cilia and basal bodies. 1. Basal bodies 2. Cilia 3. Axoneme EM 6c: monkey oviduct; longitudinal section through basal bodies and cilia. Basal body microtubules serve as assembly sites for outer doublet microtubules that are continuous with them. This is not true for the central pair of microtubules. 1. Basal body 2. Axoneme 3. Cilia EM 2b EM 10d: peripheral nerve; Schwann cells and axons. Neurofilaments (intermediate filaments) and microtubules can be seen in both myelineated and unmyelineated axons. 1 Neurofilaments 2 Microtubule EM 10c CELL COMPOSITION THREE MAJOR CLASSES OF CYTOPLASMIC STRUCTURE ------------------------------------------------- 1. MEMBRANOUS ORGANELLES 2. NON-MEMBRANOUS ORGANELLES – CYTOSKELETAL COMPONENTS etc 3. INCLUSIONS - EXPENDABLES INCLUSIONS - EXPENDABLES a. NUTRIENT e.g., GLYCOGEN, LIPID b. PIGMENT e.g., MELANIN GRANULES c. SECRETORY GRANULE e.g., ZYMOGEN GRANULE OF PANCREAS EM 2: liver. Glycogen appears as darkly staining, granular structures that are slightly larger than a ribosome. 1. Glycogen 2. Ribosome 3. Golgi vesicle 4. Lipid EM 25: ovary. Yolk protein has formed a crystalloid inclusion within the cytoplasm of this oocyte. 1. Crystalloid inclusion EM 24: corpus luteum. Several clear, non-membrane bounded lipid droplets are visible in the cytoplasm of the large cell. 1. Lipid droplets 2. Mitochondria 3. Smooth endoplasmic reticulum 4. Rough endoplasmic reticulum Slide 156: MonkeyPancreas; toulidine blue 1. Islet 2. Pancreatic acinar cells 156 Pancreas, monkey (toluidine blue) EM 1: pancreas. Secretory droplets containing digestive enzymes are major constituents of pancreatic acinar cells. 1. Zymogen granules 2. Rough endoplasmic reticulum 3. lumen EM 4 Secretory droplets EM 14: stomach. Secretory droplets with a variety of sizes and staining intensities are visible. 1. Secretory granules 2. Secretory vacuoles 3. Rough endoplasmic reticulum EM 4c: Intestinal absorptive cell (super nuclear region); 60,000x. 1. Budding RER 2. Coated vesicle 3. Golgi 4. Mitochondria 5. Nucleus 6. Plasma membrane 7. Primary lysosome EM 20: testis. Several darkly staining lipofuscin granules representing indigestible material are visible in this steroid-secreting cell. 1. Lipofuscin granule 2. Nucleus 3. Mitochondrion Lung 432 lung with bronchi 432 lung with bronchi EM 8h: Macrophage in testis; 30,000x. • Enlarged basal lamina • Heterophagic vacuole • Leydig cell cytoplasm • Nucleolus • Myoid cell EM 19c Lipid droplets EM 21: Ductus deferens; 11,000x. 1. Golgi 2. Stereocilia 3. Mitochondria 4. Swirl of SER 226 Opened Interclated disc in cardiac cells of Heart, endocardium 226 Lipofuscin in cardiac cells of Heart 146 492 492 Peripheral ganglion, monkey