University:
Texas A&M University
Course:
BIOL 319 | Integrated Human Anatomy and Physiology I
Academic year:

2021
Views:

258

Pages:

45
Author:

FrextWhestembki

Nucleus and Mitosis Structure: pancreatic acinar cells Identification: nucleus, nucleolus, heterochromatin 1. Heterochromatin 2. Nucleus 3. Nucleolus Pancreas Pancreas 32409 32409 Nuclei with nucleoli Structure: electron micrograph of hepatocyte (EM 5) Identification: nuclear envelope, inner and outer membrane, nuclear pores 1. Nuclear envelope 2. Heterochromatin 3. Euchromatin 4. Nuclear pore cytoplasm nucleus Structure: electron micrograph of stomach (EM 15) Identification: honeycomb nucleolus called nucleonema 1. Nucleus 2. Nucleonema Structure: freeze etched of small lymphocyte (EM 6) Identification: fracture plane at one of the nuclear pores jumped from the outer to the inner membrane of the nuclear envelope 1. Inner nuclear membrane 2. Outer nuclear membrane 3. Nuclear pores Mitosis anaphase Mitosis - metaphase Mitosis - anaphase Three major events ORIENTATION in mitosis ALIGNMENT SEGREGATION CHROMOSOME MOVEMENT Three contributions 3 Astral microtubules 1 2 201 Structure: intestinal absorptive cells Identification: nucleus, nucleolus 1. Nuclei 2. Nucleoli 201 Intestine crypt mitotic activity. 1. Brush border 2. Interphase nucleus 3. Anaphase 4. Artifact duodenum duodenum duodenum duodenum duodenum Ileum Cells in mitosis EM 10f: Arteriolar wall; 30,000x. 1. Apical caveolae 2. Collagen 3. Dense bodies 4. Elastic fiber 5. Endothelial cell 6. Nuclear pore 7. RER EM 19c: Sertoli cell; 13,300x. 1. Golgi 2. Heterophagic vacuole 3. Lipid droplet 4. Microtubule 5. Nuclear pores (cross-section) 6. Sertoli cell 7. Synaptoneural complex Colchicine treatment – chromosomes do not separate and NE forms around each chromosome due to breakdown of MT. 1. Multinucleated cell Cytochalasin B treatment – chromosomes separate into two normal size nuclei, but the cytoplasm does not split in two Cytochalasin B treatment-breaks down microfilaments-nucleus activity but cells do not undergo cytokinesis 1. Binucleated cell 2. Nucleolus EVENTS IN MITOSIS CYTOKINESIS Nucleus and Mitosis