Sterilization of The Room | The University of Tennessee at Martin - Edubirdie

University:
The University of Tennessee at Martin
Course:
PHCY 1213 | Pharmacy Law I
Academic year:

2024
Views:

212

Pages:

2
Author:

nicho hem

STERILIZATION OF THE ROOM I. PURPOSE To find out whether there are living or living microorganisms in an aseptic room. II. SCOPE This is carried out in the aseptic room which will be used as a room for making aseptic preparations or for making dry injection preparations. III. SPECIAL ATTENTION Testing is carried out in a sterile room IV. EQUIPMENT AND REAGENS A. Test equipment - Petri dishes : 4 pieces - Erlenmyer 500ml : 1 piece - Swabbing tool - Oven and incubator - 100ml measuring cup - Autoclave B. Reagents - Sterile distilled water - 70% Alcohol - Nutrient Agar V. PREPARATION FOR TESTING A. Sanitize the room for testing Before carrying out the test, clean the room using phenol and disinfect the LAF with 70% alcohol, then turn on the UV lamp on the LAF for 1 hour. When you want to do the test, turn off the UV lamp and turn on the fluorescent lamp and turn on the air flow. LAF is ready to use. B. Making agar media for environmental monitoring (Nutrient Agar) Weigh 23g of Nutrient Agar, dissolve it in 1L distilled water, then boil until everything is dissolved. Sterilize the agar solution in an autoclave at a temperature of 121 C for 20 minutes. C. Equipment preparation 1. Clothing, gloves, mask and shoes: Sterilized in an autoclave at a temperature of 121 C, for 20 minutes then dried in the oven at temperature 85 C, for 3 hours. 2. Glassware Each wrapped in baking paper or plain paper, then sterilize in the oven, at a temperature of 1800C for 1 hour and autoclave 121 C, for 20 minutes. VI. TESTING HOW Processing 1. Sterilize tools that will be used in the autoclave and oven 2. Prepare all the reagents needed for the Microorganism Contamination Test 3. Carry out environmental monitoring, by placing media cups for bacteria and fungi inside the LAF and outside the LAF for class 10,000 for 15 minutes 4. Close the media cup, then incubate at temperature 37 C for 24 hours 5. Interpretation of results

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