Microscopy Virtual Lab Worksheet MICROSCOPY VIRTUAL LAB WORKSHEET Begin the simulation by selecting the “Guide” button on the bottom left. 1. Which part(s) of a microscope should be held during transport? The arm of the microscope should be gripped firmly with one hand for support while placing other hand under base for support. 2. What is the purpose of a microscope’s diaphragm? It adjusts the amount of light passing through the stage opening 3. True or False: You should begin to bring your specimen into view by using the coarse focus knob first and follow this by making small adjustments to your field of view by using the fine focus knob. True 4. What magnification are oculars (aka eyepieces) typically at? What magnifications are the objective lenses typically found at? They are typically at 10x 5. What is placed on a microscope’s stage? Slides of different specimens 6. How does immersion oil affect the appearance of a specimen? You are able to see it at an increased resolution of the lens generally at 100x more. 7. What are two forms of microscope care? Lens care and storage Microscopy Virtual Lab Worksheet Continue the simulation by progressing to the “Explore” tab. Click the grey sample box on the right to begin. Select two slides from each category, excluding the “Sample Slides” category, and insert screenshots of your specimens below. Make sure to include specimen type, TOTAL magnification and a brief description of the sample in the provided space for each. ***Screenshots must be in focus!*** Plant Slides Specimen #1 Name: Plant cells Total magnification: 4x Description: The slide is completely visible at 4x with 70% fine focus 20% light adjust and 50% course focus. Specimen #2 Name: Plant Cells Total magnification: 40x Description: Microscopy Virtual Lab Worksheet The plant cell is visible at 40x you can only see a part of it, but you see more detailed structures. Animal Slides Specimen #1 Name: Spider Leg Total magnification: 10x Description: The spider leg is visible, but it is not distinguishable. Specimen #2 Name: Spider Leg Total magnification: 4x Description: The spider leg is halfway visible on the picture, but you can only see the hair follicles not the entire leg. Microscopy Virtual Lab Worksheet Although the hair follicles are easily distinguishable. Microscopy Virtual Lab Worksheet Bacteria Slides Specimen #1 Name: Acid Fast Mix Total magnification: 10x Description: The bacteria are scattered throughout the slide not detailed. Specimen #2 Name: Acid Fast Mix Total magnification: 40x Description: The bacteria are still not completely clear at this focus in regard to detailing but it is easier to look as individually rather than the whole thing. Microscopy Virtual Lab Worksheet Human Slides Specimen #1 Name: Adipose Tissue Total magnification: 4x Description: The HUMAN slide of adipose is clearly seen to be detailed you can see all of the different circles surrounding the image of the slide. Specimen #2 Name: Adipose Tissue Total magnification: 10x Description: In this slide you can see the little lines in between all of the cells and how they attach to each other at 10x.