Schlesinger Urobilin Detection: Liver Health Assessment - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

42

Pages:

2
Author:

Ethan V.

PRACTICUM II URINE CHEMICAL EXAMINATION SCHLESINGER METHOD Schlesinger Method Practicum : Schlesinger Urobilin Determination Objective : To detect liver problems Fresh urine does not contain urobilin, because urobilin is the result of oxidation of urobilinogen. If there is an examination of urobilin with fresh urine, artificial oxidation with Lugol must be carried out. Because it is a natural pigment in urine that produces a yellow color. When urine is thick, urobilin can form a reddish orange color whose intensity varies with the degree of oxidation. Method: Schlesinger Working Principle: Urobilin and Schlesinger's reagent will form a complex which can make green fluorescence or urobilinogen will be oxidized by iodine to form urobilin and if zinc acetate is added to alcohol, this urobilin will form flourescence. Tools and materials 1. Test tube 2. Pipette scale 3. Dropper pipette 4. Funnel 5. Filter paper 6. Urine sample 7. Schlesinger's reagent consisting of 2 g zinc acetate, 100 ml alcohol, shake vigorously and leave the precipitated part in the bottle 8. Lugol's solution consisting of 1g of iodine I; 2g iodide Kl ; distilled water 300ml Work procedures : 1. Put 5 ml of urine into a test tube and see whether there is green flourescence 2. If there is green fluorescence then the urine cannot be used because it will show a false (+) test result 3. If there is no green fluorescence, add 2 - 4 drops of Lugol's solution, homogenize and leave for 5 minutes 4. Add 5 ml of Schlesinger's reagent, homogenize and filter 5. Check for green fluorescence in the filtrate with sunlight reflected on a black background 6. The presence of green fluorescence indicates a positive result Notes : 1. In contrast to bilirubin, under normal circumstances urobilin is found in urine but the amount is limited, namely 4 mg/day. 2. After urine is excreted from our body, a few hours later the urobilinogen will be converted into urobilin by the presence of light. 3. Increased levels in the urine indicate liver cell damage or increased hemoglobin breakdown. When bile duct deposits occur, urobilin is not found in the urine. Interpretation of results: Negative (-): There is no green fluorescence. Positis (+): There is green fluorescence

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