Module 9: Enzyme Analysis - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

53

Pages:

2
Author:

Ethan V.

MODULE 9 ENZYME ANALYSIS Experimental Principle 1.Effect of enzyme concentration, substrate, and pH on amylase enzyme activity. The formation of a dark blue complex between starch and iodine. Starch after hydrolysis by amylase will successively form dextrin and oligosaccharide with each each level of iodine binding ability is different. Starch → Amylodextrin → Erythrodextrin → Acrodextrin → Maltose (Amil+I2) (dark blue) (red) (colorless) (colorless) 2. The effect of temperature on the activity of the amylase enzyme An increase in temperature before denaturation occurs can increase the reaction speed. However if The increase in temperature occurs when the denaturation process begins to occur, which will reduce the speed reaction. Because there are two opposing influences, an optimum point will occur, namely the most appropriate temperature for a reaction. In general, enzymes are found in animals has an optimum temperature between 40°C -50°C, while for plants it is between 50°C -60°C. Most enzymes are denatured at temperatures above 60°C. D. Tools and Materials Tool Material 1. Test tube 2. Measuring pipette 1ml, 5ml 3. Water thermometer 4. Stopwatch 5. Refrigerator 6. Tube rack 7. Basin 8. Beaker glass 9. Funnel 10. Tripod 11. Wire mesh 1. 1% starch solution 2. NaCl 1% 3. HCl 1N 4. NaOH 1N 5. Dilute iodine solution 6. Saliva 7. Ice water 8. distilled water E. Work Procedures 1. Prepare 5 test tubes, each filled with 3ml of 1% starch solution 2. In tube 1, add 1ml of 1% NaCl and 1 ml of saliva 3. In tubes 2 and 3, add 1 ml of 1N NaOH and 1 ml of saliva 4. In tube 4, add 1 ml of 1N HCl and 1 ml of saliva 5. In tube 5, add 1 ml of distilled water and 1 ml of saliva 6. Tubes 1, 2, 4, and 5 are incubated at 37°C for 10 minutes 7. Tube 3 is incubated in the refrigerator for 10 minutes 8. After all tubes have been incubated, add 3 drops of iodine and note the changes the color that occurs.

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