Enzymes Webquest Answer Key

Name: Per. Enzyme Time! WebQuest and Video Game Prior Knowledge: In this lesson you will discover how enzymes help our bodies to break down or build up a specific substrate and catalyze chemical reactions. You will learn how pH, temperature, and concentration affect the rate of chemical reactions in our bodies, and will experiment using graphs and interactive games to learn and check your understanding of enzymes. Before beginning, use the Learning Scale below to rate your knowledge of enzymes. Place a check in the Before Lesson box. Re-rate yourself in the After Lesson box after the lesson. Rating Before Lesson Rating After Lesson Learning Scale 4 I can teach others about how enzymes are specified proteins that catalyze chemical reactions and how 5 5 they can be denatured by non-optimal conditions. 3 I can explain how enzymes are specified proteins that catalyze chemical reactions and how they can be denatured by non-optimal conditions. 2 I can describe how enzymes affect chemical reactions and how they can become denatured. 1 I can identify the significance of enzymes in chemical reactions. 0 I do not understand enzymes or how they can become denatured. Task 1: Introduction to Enzymes Discover how enzymes function. Click on the following link to watch the “Enzymes and Pac-Man” video by the Amoeba Sisters. As you watch, answer the questions below. 1. What is the name of the site where the substrate binds to the enzyme? 2. What is a substrate? _ 3. What is induced fit? _The active Site The Reactant When the enzyme and substrate change slightly 4. How do enzymes change the speed of a chemical reaction? They lower the activation activity 5. What two factors can denature enzymes? extreme temperature and alkalinity 6. How is an enzyme’s shape affected when it becomes denatured? The active site an change 7. Why do lactose intolerant people get sick from drinking milk? _ Because the small intestine does not make enough digest enzyme called lactate Task 2: Enzymatic Virtual Lab Game Click on the link the below to play the “Enzymatic” virtual lab video game from the BioMan Biology website. Click “Start a New Game” to begin. Read the screens and follow the directions to complete the game. As you complete the game, answer the following questions on the handout. Part A: What Are Enzymes? - Click on “What Are Enzymes” tab. 1. Enzymes are _ that speed up or Catalyze _ proteins chemical reactions. 2. Enzymes are important because all living things must do many survive. Chemical reactions 3. The chemical reactions done by living organisms are known as Metabolism Hydrolysis 4. In biology, the two most important types of reactions are 5. Hydrolysisis a type of chemical reaction that involves the and Breakdown _ in order to _ dehydration synthesis of polymers into _ building blocks _ synthesizing Dehydration synthesis 6. _ together building blocks called is a reaction that involves _ polymers large molecules by joining 7. Dehydration synthesis happens in your cells when you need to build large molecules such as _ proteins nucleic acid , , or fats complex carbohydrates Part B: Specificity of Enzymes - Click on the “Specificity” tab. 8. Each enzyme has a unique that makes an shape 9. Each active site can only fit a specific _ _ active site substrate 10. When a substrate(s) enters the active site, the enzyme helps a specific to occur. The end result of a chemical reaction is the product Chemical reaction or products 11. What is the name of the part of the enzyme where the substrate binds and causes a chemical reaction to occur? active site 12. We say that enzymes are specific. What does this mean? Enzymes need specific substrates to work Part C: Experimenting With Enzymes – Click on “Experiments” tab. 13. Wha e factors affect an enzyme’s capability to catalyze chemical reactions? 14. Use the slider to adjust the temperature to a low (not the lowest) setting. What do you notice about the rate of , reaction when the temperature is low? The substrates slow down 15. Use the slider to adjust the temperature to a medium setting. Describe the rate of enzyme activity. go at a normal rate The substrates 16. Use the slide to adjust the temperature to the high setting. What happened to your enzyme? it means the enzyme has lost it's shape and doesn't work anymore 17. What does becoming “denatured” mean? The optimal temperature 18. What is the temperature that an enzyme works best at is called? 19. What is the optimal temperature of this enzyme? 20. The Ph_ scale 37 is used to tell how acidic or basic a solution is. 21. Temperature damage can denature an enzyme permanently. Does extreme pH changes permanently denature an It does not it can change back if it returns to its optimal enzyme? Explain. temp 22. What is the optimal pH range of the stomach enzyme pepsin? 23. Substrate _ concentration 2 refers to the relative amount of substrate in a solution. more substrate is available for the enzyme to act on _ 24. What does a high substrate concentrate mean? 25. What does a lower substrate concentration mean? less substrate is available for the enzyme to act on 26. What is your hypothesis that you typed about substrate concentration and enzyme activity? 27. Why is there more enzyme activity at a higher substrate concentration than a lower substrate concentration? because more enzymes get to attach to more substrates 28. Which factors can cause an enzyme to denature? _ low temperature or high temperature Part D: Build an Enzyme! 29. In the box to the right, draw the enzyme and substrate you will be experimenting with. 30. What is the optimal temperature of your enzyme? 30 What is the optimal pH? _ 31. What is the enzymes optimal substrate concentration? 32. Observe your machine; describe what did you see. 7 10 The substrates kept falling in place h 33. Go to the main menu and click on the “Quiz!” tab to take the quiz. What score did you receive? _83% ©2016 Science Is Real by Ana Ulrich