Sodium Homeostasis

Sodium Homeostasis ◼ 1. ◼ 1. 2. 3. 4. Sodium is an electrolyte of major importance in the human body. It is necessary for : normal extracellular volume dynamics  Na in ECF → volume contraction.  Na in ECF → edema. excitability of certain tissues cotransport and counter transport…glucose, a.a, H+ concentration of urine in thick ascending Sodium accounts for a significant portion of plasma osmolarity. The latter can be estimated by multiplying plasma sodium concentration times 2.1. Most of the primary active transport in the entire tubular system is to transport Na + Normal Renal Tubular Na+ Reabsorption 7% (16,614 mEq/day) 25,560 mEq/d 65 % (1789 mEq/d) 25 % (6390 mEq/d) 2.4% (617 mEq/d) 0.6 % (150 mEq/d) Sodium Homeostasis ◼ ◼ ◼ Sodium balance is achieved when intake and output equal each other. Sodium intake is about 120-155 mmol/d in the average American diet (≈ 4 gm). Logically, the daily output would be 120-155mmol/d as well. The kidney accounts for 115-150 mmol of this output. Hence, the kidney is a major organ in sodium homeostasis. + Na ◼ ◼ ◼ ◼ ◼ ◼ ◼ ◼ Clearence Sodium clearance can be calculated as follows: UNa+ = 150mmol/d ÷ 1.5l/d urine per day = 100mmol/l CNa+ = (UNa+ / PNa+) * V = (100 / 145) * 1 = 0.69ml/min Notice that the value is less than 1 ml/min, which indicates that sodium is mostly reabsorbed. Sodium reabsorption is rather extensive. In order to appreciate this, let’s do the math. Amount of sodium filtered per day = 180l/d * 140mM = 25200mEq…sometimes you are facing with slightly different numbers…don’t worry. Amount of sodium excreted by the kidney = 150 mM Percent reabsorbed = 25050 / 25200 = 99.4% sodium homeostasis ◼ 1. 2. 3. Three factors are principally involved in sodium homeostasis: GFR (1st factor) Aldosterone (2nd factor) Atrial natriuretic peptide (3rd factor) Na+ & H2O reabsorption occurs as the following : Segment Na+% H2O% Proximal tubule 65% 65% Descend (Henle) - 15% Ascending (Henle 25% - Distal tubule Collecting duct 7% 2.4% 10% 9% CNa+ = [UNa+ /PNa+] x V = 100/140 x 1 = < 1 ml/min Flow rate 125 ml/min <1ml/ min Prox desc asc distal coll.duct about the curve : 1. the decrement in the flow rate (F.R) throughout the kidney tubules . 2. F.R remains relatively constant at the level of the ascending limb of Henle . ◼ There are 2 ways to handle Na+ in the kidney : 1) Through filtration (↑or↓) or 2) Reabsorption (↑or↓) ◼ Example: when Na+ intake is increased: ◼ → ↑Na+ filtered → ↑reabsorption in the proximal… ◼ ◼ This is called " glomerulotubular balance " to ensure that a constant fraction is reabsorbed ( ≈ 2/3 ) → this occurs in the proximal tubules In distal tubule Na+ reabsorption is decreased. A-Reabsorption at proximal tubules ◼ 1. 2. ◼ ◼ ◼ There are 2 ways for Na+ transport through the cells: transcellular → channels (T-max)…passive at the apical side and active at the basolateral side Paracellular pathway → through the tight junctions which are not that tight in the early proximal tubules, tight junctions are not so tight → paracellular route ( + transcellular route ), so transport is NOT T-max dependent → it is gradient/time dependent . ↑[Na+] or ↑ time in TF stays in prox. tubules → more chance to be reabsorbed. In more distal parts of the nephron , the tight junctions are tighter → T-max dependent transport can be exhibited. A-Reabsorption in proximal tubules ◼ ◼ ◼ ◼ In the early part of the proximal tubule , Na+ & H2O are reabsorbed with glucose & amino acids by "cotransport process". [Na+]out = 140 mEq [Na+]in = 14 mEq So Na+ moves down gradient from the luminal side to the cell, while it is pumped actively through the basolateral membrane (anti-gradient) . A-Reabsorption in proximal tubules ◼ In the late proximal tubule , Na+ is reabsorbed with Cl- , because in the early prox.tub. , removal of large amounts of Na + creates negativity inside the lumen. so to get back to normal , Cl- is reabsorbed. Na+ follows Cl-. ◼ ◼ B. Reabsorption in descending limb of Henle (no reabsorption). C. Reabsorption in the Ascending limb of Henle. reabsorption involves ( Na+-K+-2Cl- ) cotransporter without H2O , this is called [single effect] → ↑ osmolarity in the interstitium and ↓ osmolarity in the TF (diluting segment…bcs TF become diluted) Clinical point 1. Furesamide ( Lasix): a potent loop diuretic acts on the thick ascending limb of Henle TAL where it inhibits Na-2Cl-K → ↑ Na+ and water Excretion. Lasix is used to treat pulmonary edema 2. Thiazide/Chlorothiazide (moderate diuretic) acts on distal convoluted tubule DCT inhibiting Na/Cl reabsorption ◼ Those 2 diuretics are called [potassium- wasting diuretics]…they induce hypokalemia ◼ Reabsorption of ◼ ◼ + Na D. Reabsorption in late distal tubules & cortical collecting duct. Reaborption of Na+ & secretion of K+ occur through the principal cells. Clinical point ◼ ◼ 1. Spironolactone (aldactone): is aldosterone antagonist: it decreases Na+ reabsorption and works on principal cells by decreasing K+ secretion → aldactone diuretics are called [K+ sparing diuretics]. 2. Osmotic diuretics , (ex: Mannitol): Theoretically can be considered as glomerular marker & has an osmotic effect i.e. it's not reabsorbed so it drives H2O with it , used in brain edema . Control of ◼ + Na when Na+ intake is increased → increase in GFR through increasing ECV and BP. When ECV increases → π in peritubular capillary decreases due to dilution Control of ◼ 1. 2. ◼ ◼ ◼ + Na How does the body control increase in Na+ intake ? Altering GFR Altering Reabsorption 1-Altering GFR: When Na+ intake increases → Glomerulotubular feedback does not work for unknown reason →increase Na+ Excretion. increase Na+ intake → increase Pa → increase GFR (Pressure Natriuresis) Control of Na 2-Altering reabsorption: When Na+ intake increases the RFC is shifted to the left to ensure increase Na+ excretion. This shift means less production of AII, which results in less Na+ reabsorption and thus increase its excretion. In addition less AII means less aldosterone and less Na+ reabsorption. ◼ ◼ Aldosterone is also autoregulated….means whenever [Na+] in plasma increases, Aldosterone production decreases ANP (Atrial Natriuretic Peptide) increases due to increase atrial pressure resulting in two things: 1. Afferent Arterial dilatation → increase GFR →increase Na excertion 2. inhibit adrenal cortex → decrease aldosterone production. Diuretics ◼ ◼ ◼ ◼ They are actually 7 groups each work on a specific cell and with a different mechanism. some of these groups are used for specific indications like carbonic anhydrase inhibitors which is used in glaucoma hypokalemia is a serious complication of loop diuretics and thiazide I will leave this slide to pharmacology lecture Diuretics Class Mechanism Site of Action Osmo-diuretics Mannitol loop diuretics like furosemide, ethacrynic acid and bometanide inhibit Na-K-2Cl cotransport. **Thiazide Inhibit Na-Cl cotransport.: increase Ca++ reabsorption …can be used in hypercalciuria At distal Acetazolamide (Diamox) C.A inhibitors Proximal..used for glucoma ***Spironolactone Inhibit Na+ reabsorption At principal cells Na+ channel blockers such as Amiloride and triamterene Because they inhibit Na+ reabsorption, they also inhibit K+ secretion. Therefore, they are At thick ascending Most powerful available. They increase *Ca++ and *Mg++ elimination also K+ sparing.